ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3) antibody

Antigen: ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3)

Synonyms: APG3, APG3L, PC3-96, FLJ22125, MGC15201, APG3-LIKE, DKFZp564M1178, Apg3l, Atg3l, 2610016C12Rik, Pig1, PIG-1, atg3, zgc:64156, wu:fb15g12

Clonality: Polyclonal

Host: Sheep

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN349973

Additional Information

Alternative name: autophagy-related protein 3 (ATG3, APG3, APG3L, APG3-like, hApg3, protein PC3-96)

Swiss-Prot: Q9NT62

Immunogen: A synthetic peptide as a part of human, rat, mouse autophagy-related protein 3 (ATG3, APG3, APG3L, APG3-like, hApg3, protein PC3-96) conjugated to immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential forautophagy. FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential forautophagy. SUBCELLULAR LOCATION: Cytoplasm TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. Also known as: ATG3, APG3, APG3L, APG3-like, hApg3, protein PC3-96, FLJ22125, MGC15201, DKFZp564M1178.

Specificity: Appears to be specific for ATG3.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1 : 200 to 1 : 5000. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Publications: Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Brill, Salomon, Ficarro et al.: "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." in: Analytical chemistry, Vol. 76, Issue 10, pp. 2763-72, 2004 (PubMed).