ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (C-Term) antibody

Antigen: ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7)

Synonyms: atg7, D8, UBE2, UBA1B, UBE1L, MGC12713, MOP-4, UBE1L2, FLJ10808, FLJ23367, Uba7, 1300004C08Rik, Ube1l, Ube1l2, RGD1308324, UBA7, UBE1, UBA6

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN349975

Additional Information

Alternative name: autophagy-related protein 7 (ATG7) (c-terminal region)

Swiss-Prot: O95352

Immunogen: A synthetic peptide as a part of human, rat, mouse autophagy-related protein 7 (ATG7, APG7-like, APG7L, hAGP7) conjugated to immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential forautophagy. SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. Also known as: ATG7, APG7-like, APG7L, hAGP7, APG7L, ubiquitin-activating enzyme E1-like protein.

Specificity: Appears to be specific for ATG7.

Application Details

Application Notes: IHC. Use at a dilution of 1 : 100 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Publications: Yuan, Stromhaug, Dunn: "Glucose-induced autophagy of peroxisomes in Pichia pastoris requires a unique E1-like protein." in: Molecular biology of the cell, Vol. 10, Issue 5, pp. 1353-66, 1999 (PubMed).

Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Tanida, Tanida-Miyake, Nishitani et al.: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002 (PubMed).

Mizushima, Yoshimori, Ohsumi: "Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method." in: FEBS letters, Vol. 532, Issue 3, pp. 450-4, 2002 (PubMed).

Nemoto, Tanida, Tanida-Miyake et al.: "The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39517-26, 2003 (PubMed).