coiled-coil myosin-like BCL2-interacting protein (Beclin-1, BECN1, GT197, ATG6, V) antibody

Antigen: coiled-coil myosin-like BCL2-interacting protein (Beclin-1, BECN1, GT197, ATG6, V)

Clonality: Polyclonal

Host: Sheep

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN349986

Additional Information

UniProt: Q14457

Immunogen: A synthetic peptide from human coiled-coil myosin-like BCL2-interacting protein (Beclin-1, BECN1, GT197, ATG6, VPS30) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Isotype: IgG

Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. It may play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role inautophagy. SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Note: Expressed in dendrites and cell bodies of cerebellar Purkinje cells TISSUE SPECIFICITY: Ubiquitous Also known as: Beclin-1, BECN1, GT197, ATG6, VPS30, beclin1.

Specificity: Appears to be specific for Beclin-1.

Application Details

Application Notes: IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Purification: Protein A affinity column

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Rommens, Durocher, McArthur et al.: "Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21." in: Genomics, Vol. 28, Issue 3, pp. 530-42, 1996 (PubMed).

Aita, Liang, Murty et al.: "Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." in: Genomics, Vol. 59, Issue 1, pp. 59-65, 1999 (PubMed).

Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Kihara, Kabeya, Ohsumi et al.: "Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network." in: EMBO reports, Vol. 2, Issue 4, pp. 330-5, 2001 (PubMed).

Koneri, Goi, Hirono et al.: "Beclin 1 gene inhibits tumor growth in colon cancer cell lines." in: Anticancer research, Vol. 27, Issue 3B, pp. 1453-7, 2007 (PubMed).

Lee, Jeong, Yoo et al.: "Mutational analysis of NOTCH1, 2, 3 and 4 genes in common solid cancers and acute leukemias." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 115, Issue 12, pp. 1357-63, 2008 (PubMed).