ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae) (ATG4C) antibody

Antigen: ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae) (ATG4C)

Synonyms: Apg4b, Autl1, Atg4bl, AW048066, 2510009N07Rik, APG4C, AUTL1, AUTL3, APG4-C, FLJ14867, apg4c, atg4cl, zgc:86888, wu:fc56g07, MGC148409

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN349990

Additional Information

Alternative name: cysteine protease ATG4C (autophagin-3, APG4C)

Swiss-Prot: Q96DT6

Immunogen: A synthetic peptide as a part of human, rat, mouse cysteine protease ATG4C (autophagin-3, APG4C, AUTL1, AUTL3) conjugated to immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. Cysteine protease required forautophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart, liver and testis. Also known as: ATG4C, autophagin-3, APG4C, AUTL1, AUTL3, APG4-C, ATG4autophagy related 4 homolog C (S. cerevisiae),autophagy-related protein 4 homolog C,autophagy-related cysteine endopeptidase 3, AUT-like 3 cysteine endopeptidase.

Specificity: Appears to be specific for ATG4C.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Images

WB on rat brain lysate using Rabbit antibody to human, rat, mouse cysteine protease ATG4C (autophagin-3, APG4C, AUTL1, AUTL3): whole serum (ABIN349990).

Publications

Publications: Mariuno, Uruia, Puente et al.: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).