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GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody

Details for Product No. ABIN349993, Supplier: Log in to see
Antigen
  • ATG8A
  • GABARAP
  • gabarap
  • GABARAP-a
  • mg:bb02b03
  • MM46
Epitope
N-Term
66
33
13
13
12
3
3
3
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
134
98
31
1
1
1
1
Host
Rabbit
161
19
3
Clonality
Polyclonal
Conjugate
Un-conjugated
12
12
11
5
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
144
54
52
34
31
15
12
1
1
Supplier
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Immunogen A synthetic peptide from n-terminal region of human GABARAP conjugated to an immunogenic carrier protein has been used as the antigen.
Specificity Specific for GABARAP.
Purification Whole serum
Alternative Name GABARAP (GABARAP Antibody Abstract)
Background Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. GABARAP belongs ot a large family of proteins that mediate intracellular membrane trafficking and/or fusion. GABARAP binds not only to GABA, type A but also to tubulin, gephrin, and ULK1. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. It interacts with GPHN and NSF, interacts with GABRG2, beta-tubulin and ULK1.
Subcellular location: Intracytoplasmic membrane. Cytoplasm, cytoskeleton. Note: Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.
Tissue specificity: Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Also known as: GABA(A) receptor-associated protein, GABARAP.
Research Area Proteolysis / Ubiquitin
Application Notes Use at a dilution of 1 : 100 to 1 : 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (ABIN349993) WB on 293 cell lysate using Rabbit antibody to human, rat, mouse GABA(A) receptor-ass...
Background publications Luo, Kranzler, Zuo, Zhang, Wang, Gelernter: "CHRM2 variation predisposes to personality traits of agreeableness and conscientiousness." in: Human molecular genetics, Vol. 16, Issue 13, pp. 1557-68, 2007 (PubMed).

Sou, Tanida, Komatsu, Ueno, Kominami: "Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16." in: The Journal of biological chemistry, Vol. 281, Issue 6, pp. 3017-24, 2006 (PubMed).

Boileau, Pearce, Czajkowski: "Tandem subunits effectively constrain GABAA receptor stoichiometry and recapitulate receptor kinetics but are insensitive to GABAA receptor-associated protein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 49, pp. 11219-30, 2005 (PubMed).

Tanida, Komatsu, Ueno, Kominami: "GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3." in: Biochemical and biophysical research communications, Vol. 300, Issue 3, pp. 637-44, 2002 (PubMed).

Kneussel, Haverkamp, Fuhrmann, Wang, Wässle, Olsen, Betz: "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8594-9, 2000 (PubMed).

Paz, Elazar, Fass: "Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p." in: The Journal of biological chemistry, Vol. 275, Issue 33, pp. 25445-50, 2000 (PubMed).