GABA(A) Receptor-Associated Protein-Like 2 (GABARAPL2) antibody

Antigen: GABA(A) Receptor-Associated Protein-Like 2 (GABARAPL2)

Synonyms: ATG8, GEF2, GATE16, GATE-16, Gef2, MGC108686, AI173605, MGC102013, 0610012F20Rik, 2900019O08Rik, MGC92319, zgc:92319, GABARAPL2, atg8, gef2, gate16, gate-16, MGC131197

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN349994

Additional Information

Alternative name: GABA(A) receptor-associated protein-like 2 (GABARAPL2, ATG8)

Swiss-Prot: P60520

Immunogen: A synthetic peptide as a part of human, rat, mouse GABA(A) receptor-associated protein-like 2 (GABARAPL2, ATG8) conjugated to immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association withGOSR1. SUBCELLULAR LOCATION: Golgi apparatus. TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. Also known as: FLC3A, GABARAPL2, ATG8, GEF2, GEF-2, GATE16, GATE-16, Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, General protein transport factor p16, MAP1 light chain 3-related protein.

Specificity: Appears to be specific for GABARAPL2.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1 : 300 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Publications: Okazaki, Yan, Yuasa et al.: "Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation." in: Brain research. Molecular brain research, Vol. 85, Issue 1-2, pp. 1-12, 2001 (PubMed).

Tanida, Tanida-Miyake, Nishitani et al.: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002 (PubMed).

Scherz-Shouval, Sagiv, Shorer et al.: "The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A." in: The Journal of biological chemistry, Vol. 278, Issue 16, pp. 14053-8, 2003 (PubMed).

Wiemann, Arlt, Huber et al.: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).

Sou, Tanida, Komatsu et al.: "Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16." in: The Journal of biological chemistry, Vol. 281, Issue 6, pp. 3017-24, 2006 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).