HOMER2 (Home2, Cupidin, Homer-2) (Internal Region) antibody

Antigen: HOMER2 (Home2, Cupidin, Homer-2)

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN349996

Additional Information

UniProt: Q9NSB8

Immunogen: A synthetic peptide from the internal part of human HOMER2 (Home2, Cupidin, Homer-2) conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen is homologous with the corresponding sequence in mouse, rat, bovine.

Format: Lyophilized

Description: FUNCTION: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with CENTG1. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses. SUBUNIT: Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization. SUBCELLULAR LOCATION: Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note: Postsynaptic density of neuronal cells. The stabilization and clustering of the metabotropic glutamate receptors appears to be mediated by isoform 1 and isoform 2 at the cell surface. Also known as: Home2, Homer protein homolog 2, Cupidin.

Specificity: Appears to be specific for Homer2.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Purification: Immunoaffinity purification

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Kato, Ozawa, Saitoh et al.: "Novel members of the Vesl/Homer family of PDZ proteins that bind metabotropic glutamate receptors." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 23969-75, 1998 (PubMed).

Xiao, Tu, Petralia et al.: "Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins." in: Neuron, Vol. 21, Issue 4, pp. 707-16, 1998 (PubMed).

Tu, Xiao, Yuan et al.: "Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors." in: Neuron, Vol. 21, Issue 4, pp. 717-26, 1998 (PubMed).

Soloviev, Ciruela, Chan et al.: "Molecular characterisation of two structurally distinct groups of human homers, generated by extensive alternative splicing." in: Journal of molecular biology, Vol. 295, Issue 5, pp. 1185-200, 2000 (PubMed).

Barzik, Carl, Schubert et al.: "The N-terminal domain of Homer/Vesl is a new class II EVH1 domain." in: Journal of molecular biology, Vol. 309, Issue 1, pp. 155-69, 2001 (PubMed).

Rong, Ahn, Huang et al.: "PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis." in: Nature neuroscience, Vol. 6, Issue 11, pp. 1153-61, 2003 (PubMed).