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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibody

Details for Product No. ABIN349999, Supplier: Log in to see
Antigen
  • 1010001H21Rik
  • 4922501H04Rik
  • Anx3
  • ATG8E
  • b3gnt5
  • fb12f12
  • Lc3
  • LC3
  • LC3a
  • LC3A
  • LRRGT00047
  • MAP1ALC3
  • MAP1BLC3
  • Map1lc3
  • map1lc3a
  • MAP1LC3A
  • MGC69006
  • MGC89867
  • wu:fb12f12
  • wu:fb60g11
  • wu:fe50g08
  • zgc:56434
  • zgc:77094
Reactivity
Human, Mouse (Murine), Rat (Rattus)
285
79
73
17
11
6
5
2
1
1
1
1
1
Host
Rabbit
248
31
5
4
Clonality
Polyclonal
Conjugate
Un-conjugated
23
19
18
18
13
13
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
226
144
111
61
36
27
17
14
14
12
10
6
3
Supplier
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Immunogen A synthetic peptide from human microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A, MAP1A/MAP1B LC3 A) conjugated to an immunogenic carrier protein has been used as the antigen.
Specificity Specific for MAP1LC3A.
Purification Whole serum
Alternative Name MAP1LC3A (MAP1LC3A Antibody Abstract)
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. This protein is probably involved in formation of autophagosomal vacuoles.
Subcellular location: Cytoplasm. Intracytoplasmic membrane, Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane, Lipid-anchor. Note: LC3-II binds to the autophagic membranes.
Tissue specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Also known as: MAP1A/MAP1B LC3 A, MAP1A/1B light chain 3 A, MAP1 light chain 3-like protein 1, Microtubule-associated protein 1 light chain 3 alpha,autophagy-related protein LC3 Aautophagy-related ubiquitin-like modifier LC3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A.
Research Area Cancer, Autophagy, Cytoskeleton, Proteolysis / Ubiquitin
Application Notes Use at a dilution of 1 : 100 to 1 : 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Luenemann, Schmidt, Dalakas, Muenz: "Macroautophagy as a pathomechanism in sporadic inclusion body myositis." in: Autophagy, Vol. 3, Issue 4, pp. 384-6, 2007

Pankiv, Clausen, Lamark, Brech, Bruun, Outzen, a: "p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 33, pp. 24131-45, 2007

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002