RSSA (AA 90-130) antibody

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AA 90-130
Rat (Rattus), Mouse (Murine)
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from aa region 90-130 of human RSSA conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Specificity Specific for RSSA precursor expressed on globose basal cells (GBCs).
Purification Whole serum
Background The expression of nonintegrin laminin receptor precursor protein onolfactory stem and progenitor cells has been reported by Schwob J, et al. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Also known as: Immature laminin receptor, nonintegrin laminin receptor precursor, 40S ribosomal protein SA, p40, 34/67 kDa laminin receptor, Colon carcinoma laminin-binding protein, NEM/1CHD4, Multidrug resistance-associated protein MGr1-Ag, LAMBR, LAMR1, RPSA ribosomal protein SA, LRP, 67LR, 37LRP.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Jang, Kim, Schwob: "Nonintegrin laminin receptor precursor protein is expressed on olfactory stem and progenitor cells." in: The Journal of comparative neurology, Vol. 502, Issue 3, pp. 367-81, 2007 (PubMed).

Kenmochi, Kawaguchi, Rozen, Davis, Goodman, Hudson, Tanaka, Page: "A map of 75 human ribosomal protein genes." in: Genome research, Vol. 8, Issue 5, pp. 509-23, 1998 (PubMed).

Jackers, Minoletti, Belotti, Clausse, Sozzi, Sobel, Castronovo: "Isolation from a multigene family of the active human gene of the metastasis-associated multifunctional protein 37LRP/p40 at chromosome 3p21.3." in: Oncogene, Vol. 13, Issue 3, pp. 495-503, 1996 (PubMed).

Satoh, Narumi, Sakai, Abe, Kikuchi, Matsushima, Sindoh, Motomiya: "Cloning of 67-kDa laminin receptor cDNA and gene expression in normal and malignant cell lines of the human lung." in: Cancer letters, Vol. 62, Issue 3, pp. 199-203, 1992 (PubMed).

Van den Ouweland, Van Duijnhoven, Deichmann, Van Groningen, de Leij, Van de Ven: "Characteristics of a multicopy gene family predominantly consisting of processed pseudogenes." in: Nucleic acids research, Vol. 17, Issue 10, pp. 3829-43, 1989 (PubMed).

Yow, Wong, Chen, Lee, Davis, Steele, Chen: "Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 17, pp. 6394-8, 1988 (PubMed).