Syntaxin Binding Protein 6 (STXBP6) antibody

Antigen: Syntaxin Binding Protein 6 (STXBP6)

Synonyms: LGL3, LLGL3, FLJ30922, MGC141942, MGC141968, Nbla04300, amisyn, HSPC156, FLJ39638, C85317, BC024598, MGC30948, Amisyn, MGC68796, zgc:86678, wu:fd20e06, hspc156, MGC88977, STXBP6, LOC100226852

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350011

Additional Information

Alternative name: Syntaxin-binding protein 5 (STXB5, Tomosyn-1, Llgl3)

Swiss-Prot: Q9WU70

Immunogen: A synthetic peptide as a part of rat, dog Syntaxin-binding protein 5 (STXB5, Tomosyn-1, Llgl3) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane. Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release. Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding. SUBUNIT: Part of a complex that contains STXBP5, STX4A andSNAP23. Interacts with STX1A and STX4A via its v-SNARE homology domain. Part of a complex that contains STX1, STXBP5,SNAP25 and SYT1. SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Cell junction, synapse. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles. TISSUE SPECIFICITY: Isoform 1 is detected in heart, brain, lung, liver, skeletal muscle, kidney and testis. Isoform 2 is detected in brain and in testis. Isoform 3 is detected in testis. Also known as: Lethal(2) giant larvae protein homolog 3, STXB5, Tomosyn-1, Llgl3.

Specificity: Appears to be specific for Syntaxin-binding protein 5.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Fujita, Shirataki, Sakisaka et al.: "Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process." in: Neuron, Vol. 20, Issue 5, pp. 905-15, 1998 (PubMed).

Yokoyama, Shirataki, Sakisaka et al.: "Three splicing variants of tomosyn and identification of their syntaxin-binding region." in: Biochemical and biophysical research communications, Vol. 256, Issue 1, pp. 218-22, 1999 (PubMed).

Pobbati, Razeto, Boeddener et al.: "Structural basis for the inhibitory role of tomosyn in exocytosis." in: The Journal of biological chemistry, Vol. 279, Issue 45, pp. 47192-200, 2004 (PubMed).

Baba, Sakisaka, Mochida et al.: "PKA-catalyzed phosphorylation of tomosyn and its implication in Ca2+-dependent exocytosis of neurotransmitter." in: The Journal of cell biology, Vol. 170, Issue 7, pp. 1113-25, 2005 (PubMed).