Transmembrane Protein 166 (FAM176A) antibody

Antigen: Transmembrane Protein 166 (FAM176A)

Synonyms: TMEM166, FLJ13391, Tmem166, BC014699, MGC25911, MGC37042, RGD1559797

Clonality: Polyclonal

Host: Sheep

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350019

Additional Information

Alternative name: transmembrane protein 166 (TM166, TMEM166)

UniProt: Q9H8M9

Immunogen: A synthetic peptide from human transmembrane protein 166 (TM166, TMEM166) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Acts as a regulator of programmed cell death, mediating bothautophagy and apoptosis. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Lysosome membrane; Single-pass membrane protein. TISSUE SPECIFICITY: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle. Also known as: TM166, TMEM166.

Specificity: Appears to be specific for TMEM166.

Application Details

Application Notes: IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Wan, Gong, Qin et al.: "Large-scale cDNA transfection screening for genes related to cancer development and progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 44, pp. 15724-9, 2004 (PubMed).

Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Wang, Yu, Lu et al.: "TMEM166, a novel transmembrane protein, regulates cell autophagy and apoptosis." in: Apoptosis : an international journal on programmed cell death, Vol. 12, Issue 8, pp. 1489-502, 2007 (PubMed).