Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (4th cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5)

Synonyms: TRP5, CCE2, TRP-5, Trrp5, MGC124500, TRPC5, trp, wu:fi73f08

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350021

Additional Information

Alternative name: TRPC5 (TRP5, Htrp-5, Htrp5) (4th cytoplasmic loop)

Swiss-Prot: Q9UL62

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human TRPC5 (TRP5, Htrp-5, Htrp5) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion. SUBUNIT: Interacts with NHERF. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in brain with higher levels in fetal brain. Also known as: Short transient receptor potential channel 5, TrpC5, Htrp-5, trp5, TRP5.

Specificity: Appears to be specific for TRPC5.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Zhu, Jiang, Peyton et al.: "trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry." in: Cell, Vol. 85, Issue 5, pp. 661-71, 1996 (PubMed).

Okada, Shimizu, Wakamori et al.: "Molecular cloning and functional characterization of a novel receptor-activated TRP Ca2+ channel from mouse brain." in: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10279-87, 1998 (PubMed).

Sossey-Alaoui, Lyon, Jones et al.: "Molecular cloning and characterization of TRPC5 (HTRP5), the human homologue of a mouse brain receptor-activated capacitative Ca2+ entry channel." in: Genomics, Vol. 60, Issue 3, pp. 330-40, 1999 (PubMed).

Tang, Tang, Chen et al.: "Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF." in: The Journal of biological chemistry, Vol. 275, Issue 48, pp. 37559-64, 2000 (PubMed).

Obukhov, Nowycky: "TRPC5 activation kinetics are modulated by the scaffolding protein ezrin/radixin/moesin-binding phosphoprotein-50 (EBP50)." in: Journal of cellular physiology, Vol. 201, Issue 2, pp. 227-35, 2004 (PubMed).

Ross, Grafham, Coffey et al.: "The DNA sequence of the human X chromosome." in: Nature, Vol. 434, Issue 7031, pp. 325-37, 2005 (PubMed).