HOMER1 (Home1, SYN47) (Internal Region) antibody

Antigen: HOMER1 (Home1, SYN47)

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350025

Additional Information

UniProt: Q86YM7

Immunogen: A synthetic peptide from the internal part of human HOMER1 (Home1, Homer protein homolog 1, SYN47) conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen is homologous with the corresponding sequence in mouse, rat, monkey, xenopus.

Format: Lyophilized

Description: FUNCTION: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with CENTG1. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. SUBCELLULAR LOCATION: Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note: Postsynaptic density of neuronal cells. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. Also known as: Home1, Homer protein homolog 1, SYN47.

Specificity: Appears to be specific for Homer1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Purification: Immunoaffinity purification

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Kato, Ozawa, Saitoh et al.: "vesl, a gene encoding VASP/Ena family related protein, is upregulated during seizure, long-term potentiation and synaptogenesis." in: FEBS letters, Vol. 412, Issue 1, pp. 183-9, 1997 (PubMed).

Xiao, Tu, Petralia et al.: "Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins." in: Neuron, Vol. 21, Issue 4, pp. 707-16, 1998 (PubMed).

Tu, Xiao, Yuan et al.: "Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors." in: Neuron, Vol. 21, Issue 4, pp. 717-26, 1998 (PubMed).

Roche, Tu, Petralia et al.: "Homer 1b regulates the trafficking of group I metabotropic glutamate receptors." in: The Journal of biological chemistry, Vol. 274, Issue 36, pp. 25953-7, 1999 (PubMed).

Xiao, Tu, Worley: "Homer: a link between neural activity and glutamate receptor function." in: Current opinion in neurobiology, Vol. 10, Issue 3, pp. 370-4, 2000 (PubMed).

Irie, Nakatsu, Mitsuoka et al.: "Crystal structure of the Homer 1 family conserved region reveals the interaction between the EVH1 domain and own proline-rich motif." in: Journal of molecular biology, Vol. 318, Issue 4, pp. 1117-26, 2002 (PubMed).

Saito, Kimura, Inanobe et al.: "An N-terminal sequence specific for a novel Homer1 isoform controls trafficking of group I metabotropic glutamate receptor in mammalian cells." in: Biochemical and biophysical research communications, Vol. 296, Issue 3, pp. 523-9, 2002 (PubMed).

Salanova, Priori, Barone et al.: "Homer proteins and InsP(3) receptors co-localise in the longitudinal sarcoplasmic reticulum of skeletal muscle fibres." in: Cell calcium, Vol. 32, Issue 4, pp. 193-200, 2002 (PubMed).

Sakamoto, Yoshida, Ikegami et al.: "Homer1c interacts with Hippi and protects striatal neurons from apoptosis." in: Biochemical and biophysical research communications, Vol. 352, Issue 1, pp. 1-5, 2006 (PubMed).