5-Hydroxytryptamine (Serotonin) Receptor 1A (HTR1A) (3d cytoplasmic domain) antibody

Antigen: 5-Hydroxytryptamine (Serotonin) Receptor 1A (HTR1A)

Synonyms: htr1a-A, 5-HT1A, HTR1A, 5HT1A, 5htr1a, 5-HT-1A, htr1a, Htr1a, 5-Ht1a

Epitope: 3d cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350030

Additional Information

Alternative name: 5HT1A receptor (Serotonin receptor 1A) (3d cytoplasmic domain)

Swiss-Prot: Q64264

Immunogen: A synthetic peptide from the 3d cytoplasmic domain of 5HT1A receptor (Serotonin receptor 1A, Gpcr18, 5-hydroxytryptamine receptor 1A) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 96% identity with rat and human sequence.

Format: Lyophilized

Description: FUNCTION: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Most abundantly expressed in midbrain and cortex. Also known as: Serotonin receptor 1A, Gpcr18, 5-hydroxytryptamine receptor 1A, Htr1a.

Specificity: Appears to be specific for 5HT1A receptor.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Charest, Wainer, Albert: "Cloning and differentiation-induced expression of a murine serotonin1A receptor in a septal cell line." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 12, pp. 5164-71, 1994 (PubMed).

Wilkie, Chen, Gilbert et al.: "Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors." in: Genomics, Vol. 18, Issue 2, pp. 175-84, 1994 (PubMed).

Parks, Shenk: "The serotonin 1a receptor gene contains a TATA-less promoter that responds to MAZ and Sp1." in: The Journal of biological chemistry, Vol. 271, Issue 8, pp. 4417-30, 1996 (PubMed).