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5HT1A R (AA 300-350) antibody

Details for Product No. ABIN350030
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Antigen
Epitope
AA 300-350
(1)
Reactivity
Mouse (Murine)
(1)
Host
Rabbit
(1)
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(1), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN350030
454.67 $
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Immunogen A synthetic peptide from aa region 300-350 of mouse 5HT1A R conjugated to blue carrier protein was used as the antigen. The peptide shares 96% identity with rat and human sequences.
Specificity Specific for 5HT1A receptor.
Purification Whole serum
Background Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Most abundantly expressed in midbrain and cortex. Also known as: Serotonin receptor 1A, Gpcr18, 5-hydroxytryptamine receptor 1A, Htr1a.
Application Notes A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Charest, Wainer, Albert: "Cloning and differentiation-induced expression of a murine serotonin1A receptor in a septal cell line." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 12, pp. 5164-71, 1994 (PubMed).

Wilkie, Chen, Gilbert et al.: "Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors." in: Genomics, Vol. 18, Issue 2, pp. 175-84, 1994 (PubMed).

Parks, Shenk: "The serotonin 1a receptor gene contains a TATA-less promoter that responds to MAZ and Sp1." in: The Journal of biological chemistry, Vol. 271, Issue 8, pp. 4417-30, 1996 (PubMed).

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