5HT3A receptor (5-HT3R, 5-HT-3, Serotonin receptor 3A) (1st Extracellular Domain) antibody

Antigen: 5HT3A receptor (5-HT3R, 5-HT-3, Serotonin receptor 3A)

Binding Site: 1st Extracellular Domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350031

Additional Information

UniProt: P23979

Immunogen: A synthetic peptide from the 1t extracellular domain of mouse 5HT3A receptor (5-HT3R, 5-HT-3, Serotonin receptor 3A) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 86% identity with rat sequence.

Format: Lyophilized

Description: FUNCTION: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Tissue specificity: Expressed in central and peripheral neurons. Induction: By nerve growth factor in PC12 cells. Also known as: 5-HT3R, 5-HT-3, Serotonin receptor 3A, Htr3a, 5ht3, Htr3, Serotonin gated ion channel receptor.

Specificity: Appears to be specific for 5HT3A receptor.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Maricq, Peterson, Brake et al.: "Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel." in: Science (New York, N.Y.), Vol. 254, Issue 5030, pp. 432-7, 1991 (PubMed).

Hope, Downie, Sutherland et al.: "Cloning and functional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit." in: European journal of pharmacology, Vol. 245, Issue 2, pp. 187-92, 1993 (PubMed).

Werner, Kawashima, Reid et al.: "Organization of the mouse 5-HT3 receptor gene and functional expression of two splice variants." in: Brain research. Molecular brain research, Vol. 26, Issue 1-2, pp. 233-41, 1995 (PubMed).

Uetz, Abdelatty, Villarroel et al.: "Organisation of the murine 5-HT3 receptor gene and assignment to human chromosome 11." in: FEBS letters, Vol. 339, Issue 3, pp. 302-6, 1994 (PubMed).

Kelley, Dunlop, Kirkness et al.: "A cytoplasmic region determines single-channel conductance in 5-HT3 receptors." in: Nature, Vol. 424, Issue 6946, pp. 321-4, 2003 (PubMed).