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AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473)

Details for Product anti-AKT1 Antibody No. ABIN350035, Supplier: Log in to see
Antigen
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Alternatives
anti-Human AKT antibody for Western Blotting
Epitope
pSer473
185
123
84
64
54
39
32
30
26
21
21
18
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14
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2
2
2
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2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
1
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1
1
1
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1
1
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Reactivity
Mouse (Murine), Rat (Rattus), Human
1100
615
474
61
59
54
46
35
31
29
18
9
8
5
5
3
3
3
2
2
1
1
1
1
Host
Rabbit
841
302
11
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
46
38
35
35
26
25
10
10
5
5
5
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4
4
3
3
3
3
3
3
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3
2
2
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2
2
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2
1
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1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
942
517
431
218
146
141
115
67
52
32
26
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
Options
Supplier
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Immunogen A synthetic peptide from phospho S473 of mouse AKT (RAC-alpha serine/threonine-protein kinase, PKB, RAC) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat, human and chicken.
Specificity Specific for AKT1, AKT2, AKT3.
Purification Whole serum
Alternative Name AKT (AKT1 Antibody Abstract)
Background Function: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.
Subcellular location: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.
Tissue specificity: Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis. Also known as: RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, PKB, RAC, AKT1.
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. This antibody is also very likely to recognise the unphosphorylated form.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Anai, Shojima, Katagiri, Ogihara, Sakoda, Onishi, Ono, Fujishiro, Fukushima, Horike, Viana, Kikuchi, Noguchi, Takahashi, Takata, Oka, Uchijima, Kurihara, Asano: "A novel protein kinase B (PKB)/AKT-binding protein enhances PKB kinase activity and regulates DNA synthesis." in: The Journal of biological chemistry, Vol. 280, Issue 18, pp. 18525-35, 2005 (PubMed).

Maira, Galetic, Brazil, Kaech, Ingley, Thelen, Hemmings: "Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane." in: Science (New York, N.Y.), Vol. 294, Issue 5541, pp. 374-80, 2001 (PubMed).

Pekarsky, Koval, Hallas, Bichi, Tresini, Malstrom, Russo, Tsichlis, Croce: "Tcl1 enhances Akt kinase activity and mediates its nuclear translocation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3028-33, 2000 (PubMed).

Cong, Chen, Li, Zhou, McGibbon, Taylor, Quon: "Physiological role of Akt in insulin-stimulated translocation of GLUT4 in transfected rat adipose cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 13, pp. 1881-90, 1998 (PubMed).

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