Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4)

Synonyms: TRP4, HTRP4, MGC119570, MGC119571, MGC119572, MGC119573, CCE1, Trp4, Trrp4, STRPC4, TRPC4, TRPC5, Trpc4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350036

Additional Information

Alternative name: alpha, beta, beta-2TRPC4 (TRP4)

Swiss-Prot: Q91ZM1

Immunogen: A synthetic peptide as a part of rat (and isoforms: alpha, beta, beta-2 TRPC4 (TRP4) conjugated to immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Thought to form non-selective a receptor-activated calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion. SUBUNIT: Isoform alpha but not isoform beta associates with inositol-1,4,5-triphosphate receptor (ITPR). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable). Also known as: Transient receptor potential channel 4 alpha splice variant, Trpc4_v2, Trpc4, Transient receptor potential channel 4 beta splice variant, Transient receptor potential channel 4 beta-2 splice variant, Trpc4.

Specificity: Appears to be specific for TRPC4. See also the immunogen.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Funayama, Goto, Kondo: "Cloning and expression localization of cDNA for rat homolog of TRP protein, a possible store-operated calcium (Ca2+) channel." in: Brain research. Molecular brain research, Vol. 43, Issue 1-2, pp. 259-66, 1997 (PubMed).

Tang, Tang, Chen et al.: "Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF." in: The Journal of biological chemistry, Vol. 275, Issue 48, pp. 37559-64, 2000 (PubMed).

Schaefer, Plant, Stresow et al.: "Functional differences between TRPC4 splice variants." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3752-9, 2002 (PubMed).