Amiloride-Sensitive Cation Channel 1, Neuronal (ACCN1) (C-Term) antibody

Antigen: Amiloride-Sensitive Cation Channel 1, Neuronal (ACCN1)

Synonyms: ACCN, BNC1, MDEG, ASIC2, BNaC1, ASIC2a, hBNaC1, Mdeg, ACIC2, BNaC1a, MDEG1, MDEG2, zASIC2, ACCN1, MGC143085, ASIC, ASIC1, BNaC2, ASIC1A, hBNaC2, ASIC1a, AI843610, B530003N02Rik, Asic1

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350041

Additional Information

Alternative name: amiloride sensitive cation channel 1, neuronal (ACCN1, ASIC2) (c-terminal region)

Swiss-Prot: Q16515

Immunogen: A synthetic peptide from the cytoplasmic domain of human amiloride sensitive cation channel 1, neuronal (ACCN1, ASIC2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in monkey. The antigen shares 95% identity with mouse's and rat's.

Format: Lyophilized

Description: FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM. Interacts with PRKCABP and ACCN3. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note: Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. Also known as: Amiloride-sensitive brain sodium channel, acid-sensing ion channel 2, ASIC2, brain sodium channel 1, BNaC1, BNC1, mammalian degenerin homolog, ACCN, BNAC1, MDEG.

Specificity: Appears to be specific for ACCN1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Price, Snyder, Welsh: "Cloning and expression of a novel human brain Na+ channel." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 7879-82, 1996 (PubMed).

Waldmann, Champigny, Voilley et al.: "The mammalian degenerin MDEG, an amiloride-sensitive cation channel activated by mutations causing neurodegeneration in Caenorhabditis elegans." in: The Journal of biological chemistry, Vol. 271, Issue 18, pp. 10433-6, 1996 (PubMed).

Garcuia-Aunoveros, Derfler, Neville-Golden et al.: "BNaC1 and BNaC2 constitute a new family of human neuronal sodium channels related to degenerins and epithelial sodium channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 4, pp. 1459-64, 1997 (PubMed).

Babinski, Catarsi, Biagini et al.: "Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28519-25, 2000 (PubMed).

Hruska-Hageman, Wemmie, Price et al.: "Interaction of the synaptic protein PICK1 (protein interacting with C kinase 1) with the non-voltage gated sodium channels BNC1 (brain Na+ channel 1) and ASIC (acid-sensing ion channel)." in: The Biochemical journal, Vol. 361, Issue Pt 3, pp. 443-50, 2002 (PubMed).