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Extracellular ASIC2 (AA 350-400) antibody

Details for Product No. ABIN350043, Supplier: Log in to see
Antigen
Epitope
AA 350-400
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
1
1
1
Host
Rabbit
1
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
1
1
Supplier
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Immunogen A synthetic peptide from aa region 350-400 of mouse ASIC2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in human and rat.
Specificity Specific for ACCN1.
Purification Whole serum
Background Function: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.
Subunit: Homotetramer or heterotetramer with other ASIC proteins. Interacts with STOM. Interacts with PRKCABP and ACCN3.
Subcellular location: Cell membrane, Multi-pass membrane protein. Note: Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. Also known as: Amiloride-sensitive brain sodium channel, acid-sensing ion channel 2, ASIC2, brain sodium channel 1, BNaC1, BNC1, mammalian degenerin homolog, ACCN, BNAC1, MDEG.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Hruska-Hageman, Wemmie, Price, Welsh: "Interaction of the synaptic protein PICK1 (protein interacting with C kinase 1) with the non-voltage gated sodium channels BNC1 (brain Na+ channel 1) and ASIC (acid-sensing ion channel)." in: The Biochemical journal, Vol. 361, Issue Pt 3, pp. 443-50, 2002 (PubMed).

Babinski, Catarsi, Biagini, Sueguuela: "Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28519-25, 2000 (PubMed).

Garcuia-Aunoveros, Derfler, Neville-Golden, Hyman, Corey: "BNaC1 and BNaC2 constitute a new family of human neuronal sodium channels related to degenerins and epithelial sodium channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 4, pp. 1459-64, 1997 (PubMed).

Price, Snyder, Welsh: "Cloning and expression of a novel human brain Na+ channel." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 7879-82, 1996 (PubMed).

Waldmann, Champigny, Voilley, Lauritzen, Lazdunski: "The mammalian degenerin MDEG, an amiloride-sensitive cation channel activated by mutations causing neurodegeneration in Caenorhabditis elegans." in: The Journal of biological chemistry, Vol. 271, Issue 18, pp. 10433-6, 1996 (PubMed).