ASIC1 antibody (AA 480-528)

Catalog No. ABIN350045

Product Details anti-ASIC1 Antibody

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Binding Specificity: AA 480-528

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASIC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for AISC1.

Immunogen: A synthetic peptide from aa region 480-528 of human ASIC1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Isotype: IgG

Application Details

Application Notes: A concentration of 10-50 µg/ml is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for ASIC1

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Alternative Name: ASIC1 (ACCN2 Products)

Synonyms: ACCN2 antibody, ASIC antibody, BNaC2 antibody, Accn2 antibody, ASIC1 antibody, AI843610 antibody, ASIC1a antibody, B530003N02Rik antibody, acid sensing ion channel subunit 1 antibody, acid-sensing (proton-gated) ion channel 1 antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 antibody, ASIC1 antibody, Asic1 antibody, asic1 antibody, SMARCD1 antibody

Background: This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobictransmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit, however, its function as an ion channel remains unknown.
Function: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+).
Subcellular location: Cell membrane, Multi-pass membrane protein. Note: Localizes in synaptosomes at dendritic synapses of neurons.
Tissue specificity: Expressed in dorsal root ganglia (DRG) and sciatic nerve (at protein level). Widely distributed throughout the brain. Expressed inolfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. Diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion  while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus. Also known as: Acid-sensing ion channel 1, ASIC1, Brain sodium channel 2, BNaC2, ACCN2.