Extracellular ASIC1 (AA 100-150), (Extracellular Domain) antibody

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Extracellular Domain, AA 100-150
Rat (Rattus), Mouse (Murine), Human
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from aa region 100-150 of the extracellular domain of rat ASIC1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human.
Specificity Specific for ASIC1.
Purification Whole serum
Background This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobictransmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit, however, its function as an ion channel remains unknown.
Function: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+).
Subcellular location: Cell membrane, Multi-pass membrane protein. Note=Localizes in synaptosomes at dendritic synapses of neurons.
Tissue specificity: Expressed in dorsal root ganglia (DRG) and sciatic nerve (at protein level). Widely distributed throughout the brain. Expressed inolfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. Diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion  while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus. Also known as: Acid-sensing ion channel 1, ASIC1, Brain sodium channel 2, BNaC2, ACCN2.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Coric, Zhang, Todorovic, Canessa: "The extracellular domain determines the kinetics of desensitization in acid-sensitive ion channel 1." in: The Journal of biological chemistry, Vol. 278, Issue 46, pp. 45240-7, 2003 (PubMed).

Babini, Paukert, Geisler, Grunder: "Alternative splicing and interaction with di- and polyvalent cations control the dynamic range of acid-sensing ion channel 1 (ASIC1)." in: The Journal of biological chemistry, Vol. 277, Issue 44, pp. 41597-603, 2002 (PubMed).

Ugawa, Ueda, Takahashi, Hirabayashi, Yoneda, Komai, Shimada: "Cloning and functional expression of ASIC-beta2, a splice variant of ASIC-beta." in: Neuroreport, Vol. 12, Issue 13, pp. 2865-9, 2001 (PubMed).

Voilley, de Weille, Mamet, Lazdunski: "Nonsteroid anti-inflammatory drugs inhibit both the activity and the inflammation-induced expression of acid-sensing ion channels in nociceptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 20, pp. 8026-33, 2001 (PubMed).

Baessler, Ngo-Anh, Geisler, Ruppersberg, Gruender: "Molecular and functional characterization of acid-sensing ion channel (ASIC) 1b." in: The Journal of biological chemistry, Vol. 276, Issue 36, pp. 33782-7, 2001 (PubMed).

Escoubas, De Weille, Lecoq, Diochot, Waldmann, Champigny, Moinier, Ménez, Lazdunski: "Isolation of a tarantula toxin specific for a class of proton-gated Na+ channels." in: The Journal of biological chemistry, Vol. 275, Issue 33, pp. 25116-21, 2000 (PubMed).

Askwith, Cheng, Ikuma, Benson, Price, Welsh: "Neuropeptide FF and FMRFamide potentiate acid-evoked currents from sensory neurons and proton-gated DEG/ENaC channels." in: Neuron, Vol. 26, Issue 1, pp. 133-41, 2000 (PubMed).

Chen, England, Akopian, Wood: "A sensory neuron-specific, proton-gated ion channel." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 17, pp. 10240-5, 1998 (PubMed).

Bassilana, Champigny, Waldmann, de Weille, Heurteaux, Lazdunski: "The acid-sensitive ionic channel subunit ASIC and the mammalian degenerin MDEG form a heteromultimeric H+-gated Na+ channel with novel properties." in: The Journal of biological chemistry, Vol. 272, Issue 46, pp. 28819-22, 1997 (PubMed).

Waldmann, Champigny, Bassilana, Heurteaux, Lazdunski: "A proton-gated cation channel involved in acid-sensing." in: Nature, Vol. 386, Issue 6621, pp. 173-7, 1997 (PubMed).