Extracellular ASIC1 (AA 100-150), (Extracellular Domain) antibody
Reactivity: Rat
IHC, WB
Host: Rabbit
Polyclonal
unconjugated
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- Target
- Extracellular ASIC1
- Binding Specificity
- AA 100-150, Extracellular Domain
- Reactivity
- Rat
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Specific for ASIC1.
- Cross-Reactivity
- Human, Mouse, Rat
- Cross-Reactivity (Details)
- Other species not yet tested.
- Purification
- IgG
- Immunogen
- A synthetic peptide from AA 100-150 of the extracellular domain of rat ASIC1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human.
- Isotype
- IgG
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- Application Notes
- IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
- Handling Advice
- Avoid freeze and thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
- Expiry Date
- 12 months
-
- Target
- Extracellular ASIC1
- Background
- This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit, however, its function as an ion channel remains unknown. FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). SUBCELLULAR LOCATION: Cell membrane, Multi-pass membrane protein. Note=Localizes in synaptosomes at dendritic synapses of neurons. TISSUE SPECIFICITY: Expressed in dorsal root ganglia (DRG) and sciatic nerve (at protein level). Widely distributed throughout the brain. Expressed in olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. Diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.,ACCN Channels,Acid-sensing ion channel 1, ASIC1, Brain sodium channel 2, BNaC2, ACCN2
- UniProt
- P55926
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