You are viewing an incomplete version of our website. Please click to reload the website as full version.

Amiloride-Sensitive Cation Channel 3 (ACCN3) (AA 450-490) antibody

Details for Product No. ABIN350050, Supplier: Log in to see
Antigen
  • ACCN3
  • Accn3
  • AW742291
  • DRASIC
  • SLNAC1
  • TNAC1
  • TNaC1
Epitope
AA 450-490
23
17
2
2
1
1
1
1
1
Reactivity
Human
39
11
4
1
1
Host
Rabbit
41
4
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
36
23
13
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen A synthetic peptide from aa region 450-490 of human ASIC3 conjugated to blue carrier protein was used as the antigen.
Specificity Specific for ASIC3.
Purification Whole serum
Alternative Name ASIC3 (ACCN3 Antibody Abstract)
Background Function: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.
Subunit: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and DLG4. Interacts with LIN7B, MAGI1/BAIAP1, GOPC and ACCN1.
Subcellular location: Cell membrane, Multi-pass membrane protein. Cytoplasm. Note: Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells.
Tissue specificity: Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis.
Developmental stage: Expressed in fetal tissues, expression increases in lung and kidney adult tissues. Also known as: Amiloride-sensitive cation channel 3, Acid-sensing ion channel 3, ASIC3, hASIC3, Testis sodium channel 1, hTNaC1, TNaC1, SLNAC1, ACCN3, TNAC1.
Research Area Neurology
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Catarsi, Babinski, Sueguuela: "Selective modulation of heteromeric ASIC proton-gated channels by neuropeptide FF." in: Neuropharmacology, Vol. 41, Issue 5, pp. 592-600, 2001

Babinski, Catarsi, Biagini, Sueguuela: "Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28519-25, 2000

Babinski, Lê, Séguéla: "Molecular cloning and regional distribution of a human proton receptor subunit with biphasic functional properties." in: Journal of neurochemistry, Vol. 72, Issue 1, pp. 51-7, 1999

de Weille, Bassilana, Lazdunski, Waldmann: "Identification, functional expression and chromosomal localisation of a sustained human proton-gated cation channel." in: FEBS letters, Vol. 433, Issue 3, pp. 257-60, 1998

Ishibashi, Marumo: "Molecular cloning of a DEG/ENaC sodium channel cDNA from human testis." in: Biochemical and biophysical research communications, Vol. 245, Issue 2, pp. 589-93, 1998