Amiloride-Sensitive Cation Channel 3 (ACCN3) (cytoplasmic domain) antibody

Antigen: Amiloride-Sensitive Cation Channel 3 (ACCN3)

Synonyms: ASIC3, TNaC1, DRASIC, SLNAC1, TNAC1, AW742291, Asic3, ACCN3

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350050

Additional Information

Alternative name: amiloride sensitive cation channel 3 (ACCN3, ASIC3, SLNAC1, TNAC1) (cytoplasmic domain)

Swiss-Prot: Q9UHC3

Immunogen: A synthetic peptide from the cytoplasmic domain of human amiloride sensitive cation channel 3 (ACCN3, ASIC3, SLNAC1, TNAC1) conjugated to immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and DLG4. Interacts with LIN7B, MAGI1/BAIAP1, GOPC and ACCN1. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Note: Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells. TISSUE SPECIFICITY: Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis. DEVELOPMENTAL STAGE: Expressed in fetal tissues, expression increases in lung and kidney adult tissues. Also known as: Amiloride-sensitive cation channel 3, Acid-sensing ion channel 3, ASIC3, hASIC3, Testis sodium channel 1, hTNaC1, TNaC1, SLNAC1, ACCN3, TNAC1.

Specificity: Appears to be specific for ASIC3.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Ishibashi, Marumo: "Molecular cloning of a DEG/ENaC sodium channel cDNA from human testis." in: Biochemical and biophysical research communications, Vol. 245, Issue 2, pp. 589-93, 1998 (PubMed).

de Weille, Bassilana, Lazdunski et al.: "Identification, functional expression and chromosomal localisation of a sustained human proton-gated cation channel." in: FEBS letters, Vol. 433, Issue 3, pp. 257-60, 1998 (PubMed).

Babinski, Lue, Sueguuela: "Molecular cloning and regional distribution of a human proton receptor subunit with biphasic functional properties." in: Journal of neurochemistry, Vol. 72, Issue 1, pp. 51-7, 1999 (PubMed).

Babinski, Catarsi, Biagini et al.: "Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28519-25, 2000 (PubMed).

Catarsi, Babinski, Sueguuela: "Selective modulation of heteromeric ASIC proton-gated channels by neuropeptide FF." in: Neuropharmacology, Vol. 41, Issue 5, pp. 592-600, 2001 (PubMed).