Amiloride-Sensitive Cation Channel 4, Pituitary (ACCN4) (internal) antibody

Antigen: Amiloride-Sensitive Cation Channel 4, Pituitary (ACCN4)

Synonyms: ASIC4, BNAC4, MGC17248, MGC24860, Asic4, Spasic, ACCN4

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350052

Additional Information

Alternative name: amiloride sensitive cation channel 4 (ACCN4, ASIC4) (internal region)

Swiss-Prot: Q96FT7

Immunogen: A synthetic peptide from the cytoplasmic domain of human amiloride sensitive cation channel 4 (ACCN4, ASIC4) conjugated to immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Probable cation channel with high affinity for sodium. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: This gene is predominantly expressed in the pituitary gland. Expressed in brain, spinal chord and dorsal root ganglion (DRG). Expressed by a subset of sensory neurons in the DRG. Expressed by granule cells in the cerebellar cortex. In hippocampus, expression is detected in dentate gyrus granule cells, in pyramidal cells of CA1-CA3 subfields and in interneurons of the striatum oriens and radiatum of all subfields. In cerebral cortex expressed in small, medium and large pyramidal cells in layers 2, 3 and 5 respectively. Also expressed in striatum, globus pallidus, inferior and superior calliculi, amygdala, magnocellular preoptic nucleus, islands of Calleja and large neurons ofolfactory tubercules. DEVELOPMENTAL STAGE: Highly expressed in newborn spinal chord but hardly detected in the cerebellum compared to adult. Expressed at postnatal day 1 in ependymal cells lining the central canal of spinal chord and in motor neurons. In adult, expression decreases in ependymal cells and increases in motor neurons. The number of positive interneurons decreases but the individual interneuron expression increases in adult spinal chord compared to newborn. MISCELLANEOUS: In vitro, has no proton-gated channel activity. Also known as: Amiloride sensitive cation channel 4 pituitary, acid-sensing ion channel 4, spinal chord ASIC, Spasic, BNAC4, MGC17248, MGC24860.

Specificity: Appears to be specific for ASIC4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Gruender, Geissler, Baessler et al.: "A new member of acid-sensing ion channels from pituitary gland." in: Neuroreport, Vol. 11, Issue 8, pp. 1607-11, 2000 (PubMed).

Akopian, Chen, Ding et al.: "A new member of the acid-sensing ion channel family." in: Neuroreport, Vol. 11, Issue 10, pp. 2217-22, 2000 (PubMed).