amiloride sensitive sodium channel subunitalpha (SCNN1A, SCNEA,alpha-ENaC) (1st Cytoplasmic Domain) antibody

Antigen: amiloride sensitive sodium channel subunitalpha (SCNN1A, SCNEA,alpha-ENaC)

Binding Site: 1st Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN350054

Additional Information

UniProt: Q61180

Immunogen: A synthetic peptide from the 1t cytoplasmic domain of mouse amiloride sensitive sodium channel subunit alpha (SCNN1A, SCNEA, Alpha-ENaC) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note: Apical membrane of epithelial cells. TISSUE SPECIFICITY: Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. INDUCTION: By aldosterone. Also known as: Epithelial a(+) channel subunit alpha, alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, alpha-NaCH, SCNN1, FLJ21883, ENaCalpha, Alpha-ENaC, Alpha ENaC.

Specificity: Appears to be specific for SCNN1A.

Application Details

Application Notes: IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

McDonald, Snyder, McCray et al.: "Cloning, expression, and tissue distribution of a human amiloride-sensitive Na+ channel." in: The American journal of physiology, Vol. 266, Issue 6 Pt 1, pp. L728-34, 1994 (PubMed).

Voilley, Lingueglia, Champigny et al.: "The lung amiloride-sensitive Na+ channel: biophysical properties, pharmacology, ontogenesis, and molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 1, pp. 247-51, 1994 (PubMed).

Tucker, Tamba, Lee et al.: "Cloning and functional studies of splice variants of the alpha-subunit of the amiloride-sensitive Na+ channel." in: The American journal of physiology, Vol. 274, Issue 4 Pt 1, pp. C1081-9, 1998 (PubMed).

Ludwig, Bolkenius, Wickert et al.: "Structural organisation of the gene encoding the alpha-subunit of the human amiloride-sensitive epithelial sodium channel." in: Human genetics, Vol. 102, Issue 5, pp. 576-81, 1998 (PubMed).

Arai, Zachman, Shibasaki et al.: "Polymorphisms of amiloride-sensitive sodium channel subunits in five sporadic cases of pseudohypoaldosteronism: do they have pathologic potential?" in: The Journal of clinical endocrinology and metabolism, Vol. 84, Issue 7, pp. 2434-7, 1999 (PubMed).

Chow, Wang, Plumb et al.: "Hormonal regulation and genomic organization of the human amiloride-sensitive epithelial sodium channel alpha subunit gene." in: Pediatric research, Vol. 46, Issue 2, pp. 208-14, 1999 (PubMed).

Harvey, Dinudom, Cook et al.: "The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8597-601, 2001 (PubMed).

Snyder, Olson, Thomas: "Serum and glucocorticoid-regulated kinase modulates Nedd4-2-mediated inhibition of the epithelial Na+ channel." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 5-8, 2002 (PubMed).

Saxena, Hanukoglu, Saxena et al.: "Novel mutations responsible for autosomal recessive multisystem pseudohypoaldosteronism and sequence variants in epithelial sodium channel alpha-, beta-, and gamma-subunit genes." in: The Journal of clinical endocrinology and metabolism, Vol. 87, Issue 7, pp. 3344-50, 2002 (PubMed).

McDonald, Western, McNeil et al.: "Ubiquitin-protein ligase WWP2 binds to and downregulates the epithelial Na(+) channel." in: American journal of physiology. Renal physiology, Vol. 283, Issue 3, pp. F431-6, 2002 (PubMed).