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SCNN1A antibody (Sodium Channel, Nonvoltage-Gated 1 alpha) (1st Cytoplasmic Domain)

Details for Product anti-SCNN1A Antibody No. ABIN350055, Supplier: Log in to see
Antigen
  • alphaxENaC
  • BESC2
  • besc2
  • ENaC
  • ENAC
  • ENaCa
  • enaca
  • ENaCalpha
  • mENaC
  • SCNEA
  • Scnn1
  • SCNN1
  • scnn1
  • SCNN1A
Epitope
1st Cytoplasmic Domain
35
19
15
11
6
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
47
41
28
18
Host
Rabbit
82
1
Clonality
Polyclonal
Conjugate
This SCNN1A antibody is un-conjugated
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC)
70
46
37
22
19
6
3
1
1
1
Supplier
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Immunogen A synthetic peptide from the 1t cytoplasmic domain of rat amiloride sensitive sodium channel subunit alpha (SCNN1A, SCNEA, Alpha-ENaC) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for SCNN1A.
Purification Whole serum
Alternative Name SCNN1A (SCNN1A Antibody Abstract)
Background Function: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Subcellular location: Apical cell membrane, Multi-pass membrane protein. Note: Apical membrane of epithelial cells.
Tissue specificity: Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. INDUCTION: By aldosterone. Also known as: Epithelial a(+) channel subunit alpha, alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, alpha-NaCH, SCNN1, FLJ21883, ENaCalpha, Alpha-ENaC, Alpha ENaC.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Saxena, Hanukoglu, Saxena, Thompson, Gardiner, Hanukoglu: "Novel mutations responsible for autosomal recessive multisystem pseudohypoaldosteronism and sequence variants in epithelial sodium channel alpha-, beta-, and gamma-subunit genes." in: The Journal of clinical endocrinology and metabolism, Vol. 87, Issue 7, pp. 3344-50, 2002

Snyder, Olson, Thomas: "Serum and glucocorticoid-regulated kinase modulates Nedd4-2-mediated inhibition of the epithelial Na+ channel." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 5-8, 2002

McDonald, Western, McNeil, Thomas, Olson, Snyder: "Ubiquitin-protein ligase WWP2 binds to and downregulates the epithelial Na(+) channel." in: American journal of physiology. Renal physiology, Vol. 283, Issue 3, pp. F431-6, 2002

Harvey, Dinudom, Cook, Kumar: "The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8597-601, 2001

Arai, Zachman, Shibasaki, Chrousos: "Polymorphisms of amiloride-sensitive sodium channel subunits in five sporadic cases of pseudohypoaldosteronism: do they have pathologic potential?" in: The Journal of clinical endocrinology and metabolism, Vol. 84, Issue 7, pp. 2434-7, 1999

Chow, Wang, Plumb, OBrodovich, Hu: "Hormonal regulation and genomic organization of the human amiloride-sensitive epithelial sodium channel alpha subunit gene." in: Pediatric research, Vol. 46, Issue 2, pp. 208-14, 1999

Ludwig, Bolkenius, Wickert, Marynen, Bidlingmaier: "Structural organisation of the gene encoding the alpha-subunit of the human amiloride-sensitive epithelial sodium channel." in: Human genetics, Vol. 102, Issue 5, pp. 576-81, 1998

Tucker, Tamba, Lee, Shen, Warnock, Oh: "Cloning and functional studies of splice variants of the alpha-subunit of the amiloride-sensitive Na+ channel." in: The American journal of physiology, Vol. 274, Issue 4 Pt 1, pp. C1081-9, 1998

Voilley, Lingueglia, Champigny, Mattuei, Waldmann, Lazdunski, Barbry: "The lung amiloride-sensitive Na+ channel: biophysical properties, pharmacology, ontogenesis, and molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 1, pp. 247-51, 1994

McDonald, Snyder, McCray, Welsh: "Cloning, expression, and tissue distribution of a human amiloride-sensitive Na+ channel." in: The American journal of physiology, Vol. 266, Issue 6 Pt 1, pp. L728-34, 1994