Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G) (Extracellular Domain) antibody

Antigen: Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G)

Synonyms: PHA1, ENaCg, SCNEG, ENaCgamma, ENaC, SCNN1G, scnn1g, MGC81424

Binding Site: Extracellular Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN350063

Additional Information

Alternative name: amiloride sensitive sodium channel subunit gamma (SCNN1G, SCNEG, Gamma-ENaC)

UniProt: P37091

Immunogen: A synthetic peptide from the extracellular domain of rat amiloride sensitive sodium channel subunit gamma (SCNN1G, SCNEG, Gamma-ENaC) conjugated to immunogenic carrier protein was used as the immunogen. The antigen shares 94% identity with mouse's.

Format: Lyophilized

Description: FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. SUBUNIT: Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note: Apical membrane of epithelial cells. PTM: Phosphorylated on serine and threonine residues. PTM: Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation. DISEASE: Defects in SCNN1G are a cause of Liddle syndrome. It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel. Also known as: Amiloride-sensitive sodium channel subunit gamma (SCNN1G, SCNEG), Epithelial Na(+) channel subunit gamma, Gamma-ENaC, Gamma-NaCH, PHA1, ENaCg, SCNEG, ENaCgamma.

Specificity: Appears to be specific for SCNN1G.

Application Details

Application Notes: IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Mouse IgG and Mouse Serum.

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Lingueglia, Renard, Waldmann et al.: "Different homologous subunits of the amiloride-sensitive Na+ channel are differently regulated by aldosterone." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 13736-9, 1994 (PubMed).

Kreutz, Struk, Rubattu et al.: "Role of the alpha-, beta-, and gamma-subunits of epithelial sodium channel in a model of polygenic hypertension." in: Hypertension, Vol. 29, Issue 1 Pt 1, pp. 131-6, 1997 (PubMed).

Staub, Gautschi, Ishikawa et al.: "Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination." in: The EMBO journal, Vol. 16, Issue 21, pp. 6325-36, 1998 (PubMed).

Shimkets, Lifton, Canessa: "In vivo phosphorylation of the epithelial sodium channel." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 6, pp. 3301-5, 1998 (PubMed).