Sodium Channel, Nonvoltage-Gated 1, beta (SCNN1B) (pSer633) antibody

Antigen: Sodium Channel, Nonvoltage-Gated 1, beta (SCNN1B)

Synonyms: ENaCb, SCNEB, ENaCbeta, RNENACB, SCNN1B, MGC147551, beta-ENaC, scnn1b-a, betaxENaC

Binding Site: pSer633

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN350065

Additional Information

Alternative name: amiloride-sensitive sodium channel subunit beta (SCNN1B, SCNEB) (phospho S633)

UniProt: P37090

Immunogen: A synthetic peptide to phospho S633 of rat amiloride-sensitive sodium channel subunit beta (SCNN1B, SCNEB) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous with the corresponding sequence in mouse.

Format: Lyophilized

Description: FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. SUBUNIT: Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note: Apical membrane of epithelial cells. TISSUE SPECIFICITY: Lung and kidney. Also known as: Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB, Beta-NaCH, ENaCB, ENaCbeta.

Specificity: Appears to be specific for SCNN1B.

Application Details

Application Notes: IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. Note that this antibody may also recognise the unphosphorylated form.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Kreutz, Struk, Rubattu et al.: "Role of the alpha-, beta-, and gamma-subunits of epithelial sodium channel in a model of polygenic hypertension." in: Hypertension, Vol. 29, Issue 1 Pt 1, pp. 131-6, 1997 (PubMed).

Shimkets, Lifton, Canessa: "In vivo phosphorylation of the epithelial sodium channel." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 6, pp. 3301-5, 1998 (PubMed).

Ahn, Brooker, Kosari et al.: "Cloning and functional expression of the mouse epithelial sodium channel." in: The American journal of physiology, Vol. 277, Issue 1 Pt 2, pp. F121-9, 1999 (PubMed).

Harvey, Dinudom, Cook et al.: "The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8597-601, 2001 (PubMed).

Kanelis, Rotin, Forman-Kay: "Solution structure of a Nedd4 WW domain-ENaC peptide complex." in: Nature structural biology, Vol. 8, Issue 5, pp. 407-12, 2001 (PubMed).

Fotia, Dinudom, Shearwin et al.: "The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 17, Issue 1, pp. 70-2, 2003 (PubMed).

Henry, Kanelis, OBrien et al.: "Affinity and specificity of interactions between Nedd4 isoforms and the epithelial Na+ channel." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20019-28, 2003 (PubMed).

Fotia, Ekberg, Adams et al.: "Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28930-5, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).