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AMBRA1 antibody (Autophagy/beclin-1 Regulator 1) (C-Term)

Details for Product anti-AMBRA1 Antibody No. ABIN350067, Supplier: Log in to see
Antigen
  • 2310079H06Rik
  • A130023A14
  • AA474864
  • AV021921
  • D030051N19Rik
  • DCAF3
  • mKIAA1736
  • Nyw1
  • WDR94
Epitope
C-Term
23
17
13
5
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
95
62
50
3
3
Host
Rabbit
97
Clonality
Polyclonal
Conjugate
This AMBRA1 antibody is un-conjugated
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
75
50
42
34
19
15
12
4
4
4
1
Supplier
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Supplier Product No.
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Immunogen A synthetic peptide from the c-terminal region of human AMRA1 (AMBRA1) conjugated to an immunogenic carrier protein has been used as the antigen. The peptide is homologous in mouse and rat.
Specificity Specific for AMBRA1.
Purification Whole serum
Alternative Name AMBRA1 (AMBRA1 Antibody Abstract)
Background Function: Regulatesautophagy and development of the nervous system. Involved inautophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
Subcellular location: Cytoplasmic vesicle › autophagosome Also known as: Activating molecule in BECN1-regulatedautophagy protein 1, AMBRA1, AMRA1.
Research Area Cancer, Autophagy, Neurogenesis
Application Notes Use at a dilution of 1 : 200 to 1 : 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Daub, Olsen, Bairlein, Gnad, Oppermann, Körner, Greff, Kéri, Stemmann, Mann: "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." in: Molecular cell, Vol. 31, Issue 3, pp. 438-48, 2008 (PubMed).

Dephoure, Zhou, Villén, Beausoleil, Bakalarski, Elledge, Gygi: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).

Fimia, Stoykova, Romagnoli, Giunta, Di Bartolomeo, Nardacci, Corazzari, Fuoco, Ucar, Schwartz, Gruss, Piacentini, Chowdhury, Cecconi: "Ambra1 regulates autophagy and development of the nervous system." in: Nature, Vol. 447, Issue 7148, pp. 1121-5, 2007 (PubMed).

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Nagase, Kikuno, Hattori, Kondo, Okumura, Ohara: "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 7, Issue 6, pp. 347-55, 2001 (PubMed).