AMRA1 (AMBRA1) (Internal Region) antibody

Antigen: AMRA1 (AMBRA1)

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350069

Additional Information

UniProt: Q9C0C7

Immunogen: A synthetic peptide from the internal region of human Activating molecule in BECN1 regulated autophagy protein 1 (AMBRA1, AMRA1) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in mouse.

Format: Lyophilized

Isotype: IgG

Description: Function: Regulatesautophagy and development of the nervous system. Involved inautophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. Subcellular location: Cytoplasmic vesicle › autophagosome Also known as: Activating molecule in BECN1-regulatedautophagy protein 1, AMBRA1, AMRA1.

Specificity: Appears to be specific for AMBRA1.

Application Details

Application Notes: IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Nagase, Kikuno, Hattori et al.: "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 7, Issue 6, pp. 347-55, 2001 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Fimia, Stoykova, Romagnoli et al.: "Ambra1 regulates autophagy and development of the nervous system." in: Nature, Vol. 447, Issue 7148, pp. 1121-5, 2007 (PubMed).

Dephoure, Zhou, Villén et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).

Daub, Olsen, Bairlein et al.: "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." in: Molecular cell, Vol. 31, Issue 3, pp. 438-48, 2008 (PubMed).