Ancient Ubiquitous Protein 1 (Aup1) (N-Term part) antibody

Antigen: Ancient Ubiquitous Protein 1 (Aup1)

Synonyms: AA589454, zgc:73323, zgc:77755, wu:fc37d06

Epitope: N-Term part

Clonality: Polyclonal

Host: Goat

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350072

Additional Information

Alternative name: ancient ubiquitous protein 1 (AUP1) (n-terminal part)

Swiss-Prot: A1L134

Immunogen: A synthetic pentamer-peptide from the n-terminal part of rat, mouse, human, rabbit, sheep ancient ubiquitous protein 1 (AUP1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25. Also known as: AUP1.

Specificity: Appears to be specific for AUP1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 1000 to 1: 8000 is recommended. The optimal dilution should be determined by the end user. This antibody works nicely in both paraffin and frozen sections. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Kato, Kawamata, Tamayose et al.: "Ancient ubiquitous protein 1 binds to the conserved membrane-proximal sequence of the cytoplasmic tail of the integrin alpha subunits that plays a crucial role in the inside-out signaling of alpha IIbbeta 3." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28934-41, 2002 (PubMed).