ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) antibody

Antigen: ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5)

Synonyms: ASP, APG5, APG5L, hAPG5, APG5-LIKE, apg5l

Clonality: Polyclonal

Host: Sheep

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350085

Additional Information

Alternative name: autophagy protein 5 (ATG5, ASP, APG5, APG5L, hAPG5)

Swiss-Prot: Q9H1Y0

Immunogen: A synthetic peptide as a part of human ATG5 (ASP, APG5, APG5L, hAPG5) conjugated to an immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: ATG5 is an important element forautophagy and may also play an important role in the apoptotic process. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. While the mRNA is ubiquitously transcribed its expression is highly upregulated in apoptotic cells. Also known as: APG5-like, ATG5, ASP, APG5, APG5L, hAPG5, apoptosis-specific protein.

Specificity: Appears to be specific for ATG5.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1:300 to 1: 3000. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Publications: Grand, Milner, Mustoe et al.: "A novel protein expressed in mammalian cells undergoing apoptosis." in: Experimental cell research, Vol. 218, Issue 2, pp. 439-51, 1995 (PubMed).

Hammond, Brunet, Johnson et al.: "Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast." in: FEBS letters, Vol. 425, Issue 3, pp. 391-5, 1998 (PubMed).

Mizushima, Sugita, Yoshimori et al.: "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 33889-92, 1999 (PubMed).

Schmeiser, Armstrong, Hammond et al.: "Assignment of the yeast APG5 human homologue APG5L to chromosome band 6q21 by fluorescence in situ hybridisation." in: Cytogenetics and cell genetics, Vol. 87, Issue 3-4, pp. 213-4, 2000 (PubMed).

Simonsen, Birkeland, Gillooly et al.: "Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes." in: Journal of cell science, Vol. 117, Issue Pt 18, pp. 4239-51, 2004 (PubMed).