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ATG12 antibody (Autophagy Related 12)

Details for Product anti-ATG12 Antibody No. ABIN350088, Supplier: Log in to see
Antigen
  • 4931423H11Rik
  • A330058M13Rik
  • APG12
  • Apg12l
  • APG12L
  • Atg12l
  • FBR93
  • HAPG12
Reactivity
Rat (Rattus), Mouse (Murine)
192
54
42
5
1
1
Host
Sheep
178
26
3
1
Clonality
Polyclonal
Conjugate
This ATG12 antibody is un-conjugated
10
10
10
9
9
9
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
159
95
81
39
20
11
11
9
4
4
1
Supplier
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Immunogen A synthetic peptide from mouse ATG12 conjugated to an immunogenic carrier protein has been used as the antigen.
Specificity Specific for ATG12.
Purification Whole serum
Alternative Name ATG12 (ATG12 Antibody Abstract)
Background Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved inautophagy. Following conjugation to ATG5, the conjugate participates in the formation of autophagosome. Also known as: APG12-like, HAPG12, APG12, ATG12autophagy related 12 homolog.
Application Notes Use at a dilution of 1:300 to 1: 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Prigione, Cortopassi: "Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12." in: Autophagy, Vol. 3, Issue 4, pp. 377-80, 2007

Mizushima, Kuma, Kobayashi, Yamamoto, Matsubae, Takao, Natsume, Ohsumi, Yoshimori: "Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate." in: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1679-88, 2003

Tanida, Tanida-Miyake, Komatsu, Ueno, Kominami: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002

Tanida, Nishitani, Nemoto, Ueno, Kominami: "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing." in: Biochemical and biophysical research communications, Vol. 296, Issue 5, pp. 1164-70, 2002

Mizushima, Yoshimori, Ohsumi: "Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method." in: FEBS letters, Vol. 532, Issue 3, pp. 450-4, 2002