ATG10 Autophagy Related 10 Homolog (S. Cerevisiae) (ATG10) antibody

Antigen: ATG10 Autophagy Related 10 Homolog (S. Cerevisiae) (ATG10)

Synonyms: APG10, Agp10, Apg10l, Apg10p, Atg10l, AI852123, 5330424L23Rik, 5430428K15Rik, APG10L, ATG10, MGC123341, im:7153729, zgc:123341, MGC138057, autophagy 10, Atg10

Clonality: Polyclonal

Host: Sheep

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350089

Additional Information

Alternative name: autophagy-related protein 10 (ATG10, APG10)

Swiss-Prot: Q9H0Y0

Immunogen: A synthetic peptide from human autophagy-related protein 10 (ATG10, APG10) conjugated to an immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: Autophagocytosis is a starvation-induced process responsible for transport of cytoplasmic proteins to the vacuole. FUNCTION: Plays a role inautophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule. SUBUNIT: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7. SUBCELLULAR LOCATION: Cytoplasm. Also known as: PP12616, APG10L, APG10-like, APG10, APG10L, pp12616, FLJ13954, DKFZP586I0418, ATG10autophagy related 10 homolog (S. cerevisiae).

Specificity: Appears to be specific for ATG10.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1:200 to 1: 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Publications: Mizushima, Yoshimori, Ohsumi: "Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method." in: FEBS letters, Vol. 532, Issue 3, pp. 450-4, 2002 (PubMed).

Wiemann, Arlt, Huber et al.: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).

Wan, Gong, Qin et al.: "Large-scale cDNA transfection screening for genes related to cancer development and progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 44, pp. 15724-9, 2004 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).