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Autophagy Related 10 (ATG10) antibody

Antigen

Autophagy Related 10 (ATG10)
Clonality Polyclonal
Host
Alternatives

Sheep
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
4 references available
Catalog no. ABIN350089
Quantity 150 µl  (Variants)
Price 454.67 $   Plus shipping costs $45.00
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Additional Information
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Alternative name autophagy-related protein 10 (ATG10, APG10)
UniProt Q9H0Y0
Immunogen A synthetic peptide from human autophagy-related protein 10 (ATG10, APG10) conjugated to an immunogenic carrier protein has been used as the immunogen.
Format Lyophilized
Description Autophagocytosis is a starvation-induced process responsible for transport of cytoplasmic proteins to the vacuole. FUNCTION: Plays a role inautophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule. SUBUNIT: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7. SUBCELLULAR LOCATION: Cytoplasm. Also known as: PP12616, APG10L, APG10-like, APG10, APG10L, pp12616, FLJ13954, DKFZP586I0418, ATG10autophagy related 10 homolog (S. cerevisiae).
Specificity Appears to be specific for ATG10.

Application Details
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Application Notes IHC, WB. Use at a dilution of 1:200 to 1: 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Purity whole serum
Storage Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Research Area Autophagy
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Mizushima, Yoshimori, Ohsumi: "Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method." in: FEBS letters, Vol. 532, Issue 3, pp. 450-4, 2002 (PubMed).

Wiemann, Arlt, Huber et al.: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).

Wan, Gong, Qin et al.: "Large-scale cDNA transfection screening for genes related to cancer development and progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 44, pp. 15724-9, 2004 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Autophagy Related 10 (ATG10)", type "Antibodies"
Hosts Rabbit (35), Sheep (2)
Reactivities Human (36), Mouse (Murine) (24), Rat (Rattus) (24), Cow (Bovine) (19), Dog (Canine) (1)
Applications Western Blotting (WB) (20), Immunofluorescence (IF) (16), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (4), AA 1-50 (1), AA 173-188 (1), C-Term,Ser116 (1)