Autophagy Related 10 (ATG10) antibody

Details for Product No. ABIN350089
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Antigen
Synonyms APG10, APG10L, pp12616, 5330424L23Rik, 5430428K15Rik, AI852123, Agp10, Apg10l, Apg10p, Atg10l
Reactivity
Human
(57), (25), (25), (13), (1)
Host
Sheep
(54), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(44), (19), (13), (10), (4), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 150 μL
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Catalog No. ABIN350089
454.67 $
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Immunogen A synthetic peptide from human ATG10 conjugated to an immunogenic carrier protein has been used as the antigen.
Specificity Specific for ATG10.
Purification Whole serum
Alternative Name ATG10
Background Autophagocytosis is a starvation-induced process responsible for transport of cytoplasmic proteins to the vacuole.
Function: Plays a role inautophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule.
Subunit: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7.
Subcellular location: Cytoplasm. Also known as: PP12616, APG10L, APG10-like, APG10, APG10L, pp12616, FLJ13954, DKFZP586I0418, ATG10autophagy related 10 homolog (S. cerevisiae).
Application Notes Use at a dilution of 1:200 to 1: 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Mizushima, Yoshimori, Ohsumi: "Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method." in: FEBS letters, Vol. 532, Issue 3, pp. 450-4, 2002 (PubMed).

Wiemann, Arlt, Huber et al.: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).

Wan, Gong, Qin et al.: "Large-scale cDNA transfection screening for genes related to cancer development and progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 44, pp. 15724-9, 2004 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

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