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Autophagy Related 12 (ATG12) (AA 20-70) antibody

Details for Product No. ABIN350093, Supplier: Log in to see
Antigen
  • APG12
  • APG12L
  • FBR93
  • HAPG12
  • 4931423H11Rik
  • A330058M13Rik
  • Apg12l
  • Atg12l
Epitope
AA 20-70
59
21
14
14
13
13
7
4
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Fruit Fly (Drosophila melanogaster)
185
48
39
5
1
Host
Rabbit
173
23
4
1
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
10
9
9
9
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
152
93
78
35
17
11
9
7
4
4
1
Supplier
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Immunogen A synthetic peptide from aa region 20-70 of drosophila ATG12 conjugated to blue carrier protein has been used as the antigen.
Specificity Specific for ATG12.
Purification Whole serum
Alternative Name ATG12 (ATG12 Antibody Abstract)
Background Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved inautophagy. Following conjugation to ATG5, the conjugate participates in the formation of autophagosome. Also known as: APG12-like, HAPG12, APG12, ATG12autophagy related 12 homolog.
Pathways
Application Notes Use at a dilution of 1:300 to 1: 2000.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Scott, Schuldiner, Neufeld: "Role and regulation of starvation-induced autophagy in the Drosophila fat body." in: Developmental cell, Vol. 7, Issue 2, pp. 167-78, 2004 (PubMed).

Adams, Celniker, Holt et al.: "The genome sequence of Drosophila melanogaster." in: Science (New York, N.Y.), Vol. 287, Issue 5461, pp. 2185-95, 2000 (PubMed).