Autophagy Related 12 (ATG12) (Internal Region) antibody

Antigen: Autophagy Related 12 (ATG12)

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Fruit Fly (Drosophila melanogaster)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350093

Additional Information

Alternative name: autophagy-related protein 12 (ATG12, APG12, APG12L)

UniProt: Q4V6A9

Immunogen: A synthetic peptide from the internal part of drosophila autophagy-related protein 12 (ATG12, APG12, APG12L) conjugated to an immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved inautophagy. Following conjugation to ATG5, the conjugate participates in the formation of autophagosome. Also known as: APG12-like, HAPG12, APG12, ATG12autophagy related 12 homolog.

Specificity: Appears to be specific for ATG12.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1:300 to 1: 2000. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Adams, Celniker, Holt et al.: "The genome sequence of Drosophila melanogaster." in: Science (New York, N.Y.), Vol. 287, Issue 5461, pp. 2185-95, 2000 (PubMed).

Scott, Schuldiner, Neufeld: "Role and regulation of starvation-induced autophagy in the Drosophila fat body." in: Developmental cell, Vol. 7, Issue 2, pp. 167-78, 2004 (PubMed).