Autophagy Related 12 (ATG12) (Isoform 1, Isoform 2) antibody

Antigen: Autophagy Related 12 (ATG12)

Binding Site: Isoform 1, Isoform 2

Clonality: Polyclonal

Host: Sheep

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350096

Additional Information

Alternative name: autophagy-related protein 12,(ATG12, APG12, APG12L, HAPG12)

UniProt: O94817

Immunogen: A synthetic peptide as a part of human ATG12, isoforms 1 and 2 (APG12, APG12L, HAPG12) conjugated to an immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved inautophagy. Following conjugation to ATG5, the conjugate participates in the formation of autophagosome. Also known as: APG12-like, HAPG12, APG12, ATG12autophagy related 12 homolog.

Specificity: Appears to be specific for ATG12.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1:300 to 1: 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Tanida, Nishitani, Nemoto et al.: "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing." in: Biochemical and biophysical research communications, Vol. 296, Issue 5, pp. 1164-70, 2002 (PubMed).

Mizushima, Yoshimori, Ohsumi: "Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method." in: FEBS letters, Vol. 532, Issue 3, pp. 450-4, 2002 (PubMed).

Mizushima, Kuma, Kobayashi et al.: "Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate." in: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1679-88, 2003 (PubMed).

Prigione, Cortopassi: "Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12." in: Autophagy, Vol. 3, Issue 4, pp. 377-80, 2007 (PubMed).