Growth Associated Protein 43 (GAP43) (pSer41) antibody

Antigen: Growth Associated Protein 43 (GAP43)

Synonyms: B-50, PP46, Basp2, GAP-43, gap-43, MGC64520

Binding Site: pSer41

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN350109

Additional Information

Alternative name: Axonal membrane protein GAP-43 (Neuromodulin, growth-associated protein 43)

UniProt: P17677

Immunogen: A synthetic peptide to phospho S41 of human Axonal membrane protein GAP-43 (Neuromodulin, growth-associated protein 43) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many species including rat, mouse and zebrafish.

Format: Lyophilized

Description: FUNCTION: This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes. Also known as: Neuromodulin, growth-associated protein 43, protein F1, Basp2, B-50.

Specificity: Appears to be specific for GAP-43.

Application Details

Application Notes: IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IHC on rat spinal cord (dorsal horn) using Rabbit antibody to rat, mouse Axonal membrane protein GAP-43 (Neuromodulin, growth-associated protein 43): whole serum at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Publications

Cimler, Giebelhaus, Wakim et al.: "Characterization of murine cDNAs encoding P-57, a neural-specific calmodulin-binding protein." in: The Journal of biological chemistry, Vol. 262, Issue 25, pp. 12158-63, 1987 (PubMed).

Ballif, Villén, Beausoleil et al.: "Phosphoproteomic analysis of the developing mouse brain." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 11, pp. 1093-101, 2004 (PubMed).

Vosseller, Hansen, Chalkley et al.: "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." in: Proteomics, Vol. 5, Issue 2, pp. 388-98, 2005 (PubMed).

Trinidad, Specht, Thalhammer et al.: "Comprehensive identification of phosphorylation sites in postsynaptic density preparations." in: Molecular & cellular proteomics : MCP, Vol. 5, Issue 5, pp. 914-22, 2006 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).