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GAP43 antibody (Growth Associated Protein 43) (pSer41)

Details for Product anti-GAP43 Antibody No. ABIN350111, Supplier: Log in to see
Antigen
  • Basp2
  • B-50
  • PP46
  • GAP-43
  • cb310
  • gap
  • gap43
  • gap43a
  • pp46
  • b-50
  • gap-43
  • gap43b
Epitope
pSer41
49
23
18
13
11
7
6
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
170
152
122
25
12
10
9
9
8
6
5
5
3
2
2
1
1
Host
Sheep
176
39
7
6
1
Clonality
Polyclonal
Conjugate
This GAP43 antibody is un-conjugated
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC)
137
64
59
43
41
35
28
24
22
3
3
1
Supplier
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Immunogen A synthetic peptide to phospho S41 of human Axonal membrane protein GAP-43 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat, mouse, zebra fish and chicken.
Specificity Specific for GAP-43.
Purification Whole serum
Alternative Name GAP 43 (GAP43 Antibody Abstract)
Background Function: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons.
Subunit: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.
Subcellular location: Cell membrane, Peripheral membrane protein, Cytoplasmic side. Cell projection, growth cone membrane, Peripheral membrane protein, Cytoplasmic side. Cell junction, synapse. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes. Also known as: Neuromodulin, growth-associated protein 43, protein F1, Basp2, B-50.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications. This antibody may also recognise the unphosphorylated form.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-GAP43 antibody (Growth Associated Protein 43) (pSer41) (ABIN350111) WB on rat brain lysate using Sheep antibody to phospho S41 of Axonal membrane protein...
Background publications Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).

Trinidad, Specht, Thalhammer et al.: "Comprehensive identification of phosphorylation sites in postsynaptic density preparations." in: Molecular & cellular proteomics : MCP, Vol. 5, Issue 5, pp. 914-22, 2006 (PubMed).

Vosseller, Hansen, Chalkley et al.: "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." in: Proteomics, Vol. 5, Issue 2, pp. 388-98, 2005 (PubMed).

Ballif, Villén, Beausoleil et al.: "Phosphoproteomic analysis of the developing mouse brain." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 11, pp. 1093-101, 2004 (PubMed).

Cimler, Giebelhaus, Wakim et al.: "Characterization of murine cDNAs encoding P-57, a neural-specific calmodulin-binding protein." in: The Journal of biological chemistry, Vol. 262, Issue 25, pp. 12158-63, 1987 (PubMed).