Bassoon (Presynaptic Cytomatrix Protein) (BSN) (internal) antibody

Antigen: Bassoon (Presynaptic Cytomatrix Protein) (BSN)

Synonyms: BSN, ZNF231, Znf231

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350115

Additional Information

Alternative name: Bassoon (Zinc finger protein 231, BSN) (internal region)

Swiss-Prot: Q9UPA5

Immunogen: A synthetic peptide from internal part of human Bassoon (Zinc finger protein 231, BSN) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. SUBCELLULAR LOCATION: Cytoplasm. Cell junction, synapse, synaptosome. Cytoplasm, cytoskeleton. TISSUE SPECIFICITY: Exclusively expressed in brain. Also known as: Protein bassoon, BSN, Zinc finger protein 231.

Specificity: Appears to be specific for BSN.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Ishikawa, Nagase, Nakajima et al.: "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 4, Issue 5, pp. 307-13, 1998 (PubMed).

Hashida, Goto, Zhao et al.: "Cloning and mapping of ZNF231, a novel brain-specific gene encoding neuronal double zinc finger protein whose expression is enhanced in a neurodegenerative disorder, multiple system atrophy (MSA)." in: Genomics, Vol. 54, Issue 1, pp. 50-8, 1999 (PubMed).

Khidekel, Ficarro, Peters et al.: "Exploring the O-GlcNAc proteome: direct identification of O-GlcNAc-modified proteins from the brain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 36, pp. 13132-7, 2004 (PubMed).

Yu, Zhu, Chan et al.: "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." in: Journal of proteome research, Vol. 6, Issue 11, pp. 4150-62, 2007 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).