Actin (C-Term) antibody

Details for Product No. ABIN350117
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B,  ... show more
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B, mus79B, Act(88F), Act88-F, Act88f, CG5178, Dmel\\CG5178, E, Ifm(3)7, act 88F, act88F, rsd, Act87e, CG18290, Dmel\\CG18290, act 87E, act87E, mus87E, Act88F, Dsim\\GD19025, GD19025, dsim_GLEANR_2829, 42A, A, AFFX-Dros-ACTIN_M_r_at, Act, Act42, Act42a, Actin/BAP47, BAP47, CG12051, Dmel\\CG12051, act 42A, act42A show less
Epitope
C-Term
(16), (8), (5), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Zebrafish
(107), (64), (64), (43), (20), (15), (15), (13), (8), (7), (6), (6), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(92), (57), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(105), (48), (47), (37), (29), (25), (20), (18), (13), (2), (1), (1)
Pubmed 5 references available
Catalog no. ABIN350117
Quantity 100 µL
Price
454.67 $   Plus shipping costs $45.00
Options
Shipping to (Change)
Availability Will be delivered in 7 to 8 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide from the c-terminal of human beta, gamma, kappa Actin (ACTB, ACTG, ACTK) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in many species including rat, mouse, zebra fish and xenopus.
Specificity Specific for TRPA1.
Purification whole serum
Background Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Subcellular location: Cytoplasm, cytoskeleton. Also known as: ACTB, ACTG, ACTK.
UniProt Q5UM15
Research Area Neurology
Application Notes A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Liquid
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage -20 °C
Storage Comment Maintain the reconstituted antibodies frozen at -20 °C for long term storage and refrigerated at 2-8 °C for a shorter term.
Expiry Date 12 months
Supplier Images
anti-Actin (C-Term) antibody WB on rat brain lysate using Rabbit antibody to c-terminal of beta, gamma, kappa Actin (ACTB, ACTG, ACTK): whole serum (ABIN350117) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.
General Erba, Gunning, Kedes: "Nucleotide sequence of the human gamma cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes." in: Nucleic acids research, Vol. 14, Issue 13, pp. 5275-94, 1986 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Procaccio, Salazar, Ono et al.: "A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia." in: American journal of human genetics, Vol. 78, Issue 6, pp. 947-60, 2006 (PubMed).

Chang, Yang, Lai et al.: "Identification of a novel actin isoform in hepatocellular carcinoma." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 36, Issue 1, pp. 33-9, 2006 (PubMed).

Zahedi, Lewandrowski, Wiesner et al.: "Phosphoproteome of resting human platelets." in: Journal of proteome research, Vol. 7, Issue 2, pp. 526-34, 2008 (PubMed).

Hosts (92), (57), (2), (1)
Reactivities (107), (64), (64), (43), (20), (15), (15), (13), (8), (7), (6), (6), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (105), (48), (47), (37), (29), (25), (20), (18), (13), (2), (1), (1)
Conjugates (9), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Epitopes (16), (8), (5), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top