beta, gamma, kappa Actin (ACTB, ACTG, ACTK) (C-Term) antibody

Antigen: beta, gamma, kappa Actin (ACTB, ACTG, ACTK)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350117

Additional Information

UniProt: P60709

Immunogen: A synthetic peptide from the c-terminal of human beta, gamma, kappa Actin (ACTB, ACTG, ACTK) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in many species including rat, mouse, zebra fish and xenopus.

Format: Lyophilized

Description: FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Also known as: ACTB, ACTG, ACTK.

Specificity: Appears to be specific for Actin.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on rat brain lysate using Rabbit antibody to c-terminal of beta, gamma, kappa Actin (ACTB, ACTG, ACTK): whole serum (ABIN350117) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Erba, Gunning, Kedes: "Nucleotide sequence of the human gamma cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes." in: Nucleic acids research, Vol. 14, Issue 13, pp. 5275-94, 1986 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Procaccio, Salazar, Ono et al.: "A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia." in: American journal of human genetics, Vol. 78, Issue 6, pp. 947-60, 2006 (PubMed).

Chang, Yang, Lai et al.: "Identification of a novel actin isoform in hepatocellular carcinoma." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 36, Issue 1, pp. 33-9, 2006 (PubMed).

Zahedi, Lewandrowski, Wiesner et al.: "Phosphoproteome of resting human platelets." in: Journal of proteome research, Vol. 7, Issue 2, pp. 526-34, 2008 (PubMed).