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Actin antibody (C-Term)

Details for Product anti-Actin Antibody No. ABIN350118, Supplier: Log in to see
Antigen
  • 42A
  • 143060_f_at
  • A
  • Act
  • Act(88F)
  • Act42
  • act42A
  • act 42A
  • Act42a
  • Act57
  • act57A
  • Act57A
  • act 57A
  • Act57b
  • act57B
  • act57b
  • act 79B
  • act79B
  • Act87e
  • act 87E
  • act87E
  • Act88-F
  • Act88F
  • act88F
  • act 88F
  • Act88f
  • Actin
  • actin
  • Actin/BAP47
  • AFFX-Dros-ACTIN_M_r_at
  • anon-EST:CL2c12
  • BAP47
  • BEST:RE38067
  • C
  • CG5178
  • CG7478
  • CG10067
  • CG12051
  • CG18290
  • CG30294
  • cytoplasmic
  • D
  • Dmel\\CG5178
  • Dmel\\CG7478
  • Dmel\\CG10067
  • Dmel\\CG12051
  • Dmel\\CG18290
  • Dsim\\GD19025
  • dsim_GLEANR_2829
  • E
  • GD19025
  • Ifm(3)7
  • M
  • mus79B
  • mus87E
  • rsd
  • T10
Epitope
C-Term
37
30
20
13
13
8
5
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
277
201
135
123
74
49
33
28
27
21
19
18
18
16
16
16
15
15
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
228
98
5
2
Clonality
Polyclonal
Conjugate
This Actin antibody is un-conjugated
13
10
9
7
7
7
6
6
6
6
6
6
6
6
6
6
6
3
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
256
145
121
78
76
76
69
62
55
11
5
4
3
1
1
1
Supplier
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Immunogen A synthetic peptide from the c-terminal of beta, gamma, kappa Actin (ACTB, ACTG, ACTK) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, zebra fish and xenopus.
Isotype IgG
Specificity Specific for Actin.
Background Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Subcellular location: Cytoplasm, cytoskeleton. Also known as: ACTB, ACTG, ACTK.
Research Area Cancer, Apoptosis/Necrosis, Cytoskeleton, Microfilaments, Extracellular Matrix
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Zahedi, Lewandrowski, Wiesner, Wortelkamp, Moebius, Schütz, Walter, Gambaryan, Sickmann: "Phosphoproteome of resting human platelets." in: Journal of proteome research, Vol. 7, Issue 2, pp. 526-34, 2008 (PubMed).

Chang, Yang, Lai, Yeh, Chen, Yeh: "Identification of a novel actin isoform in hepatocellular carcinoma." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 36, Issue 1, pp. 33-9, 2006 (PubMed).

Procaccio, Salazar, Ono, Styers, Gearing, Davila, Jimenez, Juncos, Gutekunst, Meroni, Fontanella, Sontag, Sontag, Faundez, Wainer: "A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia." in: American journal of human genetics, Vol. 78, Issue 6, pp. 947-60, 2006 (PubMed).

Gevaert, Goethals, Martens, Van Damme, Staes, Thomas, Vandekerckhove: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Erba, Gunning, Kedes: "Nucleotide sequence of the human gamma cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes." in: Nucleic acids research, Vol. 14, Issue 13, pp. 5275-94, 1986 (PubMed).