You are viewing an incomplete version of our website. Please click to reload the website as full version.

Actin antibody (C-Term)

Details for Product anti-Actin Antibody No. ABIN350119, Supplier: Log in to see
Antigen
  • Act57
  • Act57A
  • Act57b
  • Actin
  • BEST:RE38067
  • C
  • CG10067
  • CG30294
  • Dmel\\CG10067
  • M
  • T10
  • act 57A
  • act57A
  • act57B
  • act57b
  • actin
  • anon-EST:CL2c12
  • 143060_f_at
  • CG7478
  • D
  • Dmel\\CG7478
  • act 79B
  • act79B
  • mus79B
  • Act(88F)
  • Act88-F
  • Act88f
  • CG5178
  • Dmel\\CG5178
  • E
  • Ifm(3)7
  • act 88F
  • act88F
  • rsd
  • Act87e
  • CG18290
  • Dmel\\CG18290
  • act 87E
  • act87E
  • mus87E
  • Act88F
  • Dsim\\GD19025
  • GD19025
  • dsim_GLEANR_2829
  • 42A
  • A
  • AFFX-Dros-ACTIN_M_r_at
  • Act
  • Act42
  • Act42a
  • Actin/BAP47
  • BAP47
  • CG12051
  • Dmel\\CG12051
  • act 42A
  • act42A
  • cytoplasmic
Epitope
C-Term
34
26
13
13
8
5
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
142
72
67
48
13
12
9
5
5
5
5
5
5
4
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Sheep
96
94
4
2
Clonality
Polyclonal
Conjugate
This Actin antibody is un-conjugated
8
5
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
147
55
49
49
46
34
28
12
12
4
3
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Immunogen A synthetic peptide from the c-terminal of human beta, gamma, kappa Actin (ACTB, ACTG, ACTK) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, zebra fish and xenopus.
Specificity Specific for Actin.
Purification Whole serum
Background Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Subcellular location: Cytoplasm, cytoskeleton. Also known as: ACTB, ACTG, ACTK.
Research Area Cancer, Apoptosis/Necrosis, Cytoskeleton, Microfilaments, Extracellular Matrix
Pathways
Application Notes A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Zahedi, Lewandrowski, Wiesner et al.: "Phosphoproteome of resting human platelets." in: Journal of proteome research, Vol. 7, Issue 2, pp. 526-34, 2008 (PubMed).

Chang, Yang, Lai et al.: "Identification of a novel actin isoform in hepatocellular carcinoma." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 36, Issue 1, pp. 33-9, 2006 (PubMed).

Procaccio, Salazar, Ono et al.: "A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia." in: American journal of human genetics, Vol. 78, Issue 6, pp. 947-60, 2006 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Erba, Gunning, Kedes: "Nucleotide sequence of the human gamma cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes." in: Nucleic acids research, Vol. 14, Issue 13, pp. 5275-94, 1986 (PubMed).