Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) (Cytoplasmic Domain) antibody

Antigen: Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A)

Synonyms: EA2, FHM, MHP, APCA, HPCA, MHP1, SCA6, CAV2.1, CACNL1A4, la, tg, rkr, Caca1a, Cav2.1, Ccha1a, leaner, nmf352, rocker, alpha1A, Cacnl1a4, tottering, BccA1, rbA-1, cav2.1, cacna-a, ca(v)2.1, si:ch211-237k17.1, CACNA1A, CACH4, CACN3

Binding Site: Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350134

Additional Information

Alternative name: CACNA1A (Cav2.1)

UniProt: O00555

Immunogen: A synthetic peptide from a cytoplasmic domain of human CACNA1A (Brain calcium channel I, Cav2.1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 87% identity with mouse and rat sequence.

Format: Lyophilized

Description: FUNCTION: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by the funnel toxin (Ftx) and by the omega-agatoxin-IVA (omega-Aga-IVA). They are however insensitive to dihydropyridines (DHP), and omega-conotoxin-GVIA (omega-CTx-GVIA). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Tissue specificity: Brain specific; mainly found in cerebellum, cerebral cortex, thalamus and hypothalamus. No expression in heart, kidney, liver or muscle. Purkinje cells contain predominantly P-type VSCC, the Q-type being a prominent calcium current in cerebellar granule cells. Also known as: BI, Brain calcium channel I, Cach4, Cacn3, Cacna1a, Cacnl1a4, Calcium channel L type alpha-1 polypeptide, Voltage-dependent P/Q-type calcium channel alpha-1A subunit, Voltage-gated calcium channel alpha subunit Cav2.1.

Specificity: Appears to be specific for CACNA1A.

Synonyms: EA2, FHM, MHP, APCA, HPCA, MHP1, SCA6, CAV2.1, CACNL1A4, la, tg, rkr, Caca1a, Cav2.1, Ccha1a, leaner, nmf352, rocker, alpha1A, Cacnl1a4, tottering, BccA1, rbA-1, cav2.1, cacna-a, ca(v)2.1, si:ch211-237k17.1, CACNA1A, CACH4, CACN3

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Barry, Viglione, Kim et al.: "Expression and antibody inhibition of P-type calcium channels in human small-cell lung carcinoma cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 1 Pt 1, pp. 274-83, 1995 (PubMed).

Ophoff, Terwindt, Vergouwe et al.: "Familial hemiplegic migraine and episodic ataxia type-2 are caused by mutations in the Ca2+ channel gene CACNL1A4." in: Cell, Vol. 87, Issue 3, pp. 543-52, 1996 (PubMed).

Hans, Urrutia, Deal et al.: "Structural elements in domain IV that influence biophysical and pharmacological properties of human alpha1A-containing high-voltage-activated calcium channels." in: Biophysical journal, Vol. 76, Issue 3, pp. 1384-400, 1999 (PubMed).

Ducros, Denier, Joutel et al.: "The clinical spectrum of familial hemiplegic migraine associated with mutations in a neuronal calcium channel." in: The New England journal of medicine, Vol. 345, Issue 1, pp. 17-24, 2001 (PubMed).

Mantuano, Veneziano, Spadaro et al.: "Clusters of non-truncating mutations of P/Q type Ca2+ channel subunit Ca(v)2.1 causing episodic ataxia 2." in: Journal of medical genetics, Vol. 41, Issue 6, pp. e82, 2004 (PubMed).