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Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) (Cytoplasmic Domain) antibody
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||CACNA1A (Cav2.1)|
|Immunogen||A synthetic peptide from a cytoplasmic domain of human CACNA1A (Brain calcium channel I, Cav2.1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 87% identity with mouse and rat sequence.|
|Description||FUNCTION: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by the funnel toxin (Ftx) and by the omega-agatoxin-IVA (omega-Aga-IVA). They are however insensitive to dihydropyridines (DHP), and omega-conotoxin-GVIA (omega-CTx-GVIA). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Tissue specificity: Brain specific; mainly found in cerebellum, cerebral cortex, thalamus and hypothalamus. No expression in heart, kidney, liver or muscle. Purkinje cells contain predominantly P-type VSCC, the Q-type being a prominent calcium current in cerebellar granule cells. Also known as: BI, Brain calcium channel I, Cach4, Cacn3, Cacna1a, Cacnl1a4, Calcium channel L type alpha-1 polypeptide, Voltage-dependent P/Q-type calcium channel alpha-1A subunit, Voltage-gated calcium channel alpha subunit Cav2.1.|
|Specificity||Appears to be specific for CACNA1A.|
|Synonyms||EA2, FHM, MHP, APCA, HPCA, MHP1, SCA6, CAV2.1, CACNL1A4, la, tg, rkr, Caca1a, Cav2.1, Ccha1a, leaner, nmf352, rocker, alpha1A, Cacnl1a4, tottering, BccA1, rbA-1, cav2.1, cacna-a, ca(v)2.1, si:ch211-237k17.1, CACNA1A, CACH4, CACN3|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Barry, Viglione, Kim et al.: "Expression and antibody inhibition of P-type calcium channels in human small-cell lung carcinoma cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 1 Pt 1, pp. 274-83, 1995 (PubMed).
Ophoff, Terwindt, Vergouwe et al.: "Familial hemiplegic migraine and episodic ataxia type-2 are caused by mutations in the Ca2+ channel gene CACNL1A4." in: Cell, Vol. 87, Issue 3, pp. 543-52, 1996 (PubMed).
Hans, Urrutia, Deal et al.: "Structural elements in domain IV that influence biophysical and pharmacological properties of human alpha1A-containing high-voltage-activated calcium channels." in: Biophysical journal, Vol. 76, Issue 3, pp. 1384-400, 1999 (PubMed).
Ducros, Denier, Joutel et al.: "The clinical spectrum of familial hemiplegic migraine associated with mutations in a neuronal calcium channel." in: The New England journal of medicine, Vol. 345, Issue 1, pp. 17-24, 2001 (PubMed).
Mantuano, Veneziano, Spadaro et al.: "Clusters of non-truncating mutations of P/Q type Ca2+ channel subunit Ca(v)2.1 causing episodic ataxia 2." in: Journal of medical genetics, Vol. 41, Issue 6, pp. e82, 2004 (PubMed).
|Reactivities||Rat (Rattus) (23), Cow (Bovine) (19), Human (19), Mouse (Murine) (19), Pig (Porcine) (19)|
|Applications||Immunofluorescence (IF) (16), Western Blotting (WB) (4), ELISA (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|