Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (cytoplasmic domain) antibody

Antigen: Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C)

Synonyms: TS, CACH2, CACN2, CaV1.2, CCHL1A1, CACNL1A1, MGC120730, Cav1.2, Cchl1a1, RATIVS302, isl, C-LTCC, CACNA1C, ICA, Cacna1c, cav1.2, ca(v)1.2

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350136

Additional Information

Alternative name: CACNA1C (Cav1.2) (cytoplasmic domain)

Swiss-Prot: Q13936

Immunogen: A synthetic peptide from a cytoplasmic domain of human CACNA1C (Cav1.2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 96% identity with mouse, rat and rabbit sequence.

Format: Lyophilized

Description: Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Subcellular location: Membrane; Multi-pass membrane protein. Tissue specificity: Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle. Also known as: CACH2, CACN2, CACNL1A1, CCHL1A1, Voltage-gated calcium channel subunit alpha Cav1.2, Calcium channel, L type, alpha-1 polypeptide, cardiac muscle, Voltage dependent L-type calcium channel subunit alpha-1C.

Specificity: Appears to be specific for CACNA1C.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Soldatov, Bouron, Reuter: "Different voltage-dependent inhibition by dihydropyridines of human Ca2+ channel splice variants." in: The Journal of biological chemistry, Vol. 270, Issue 18, pp. 10540-3, 1995 (PubMed).

Schultz, Mikala, Yatani et al.: "Cloning, chromosomal localization, and functional expression of the alpha 1 subunit of the L-type voltage-dependent calcium channel from normal human heart." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 13, pp. 6228-32, 1993 (PubMed).

Zuehlke, Bouron, Soldatov et al.: "Ca2+ channel sensitivity towards the blocker isradipine is affected by alternative splicing of the human alpha1C subunit gene." in: FEBS letters, Vol. 427, Issue 2, pp. 220-4, 1998 (PubMed).

Scherer, Muzny, Buhay et al.: "The finished DNA sequence of human chromosome 12." in: Nature, Vol. 440, Issue 7082, pp. 346-51, 2006 (PubMed).

Antzelevitch, Pollevick, Cordeiro et al.: "Loss-of-function mutations in the cardiac calcium channel underlie a new clinical entity characterized by ST-segment elevation, short QT intervals, and sudden cardiac death." in: Circulation, Vol. 115, Issue 4, pp. 442-9, 2007 (PubMed).