CACNA1D (Cav1.3) (cytoplasmic domain) antibody

Antigen: CACNA1D (Cav1.3)

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350138

Additional Information

Alternative name: CACNA1D (Cav1.3) (cytoplasmic domain)

Swiss-Prot: Q01668

Immunogen: A synthetic peptide from the a cytoplasmic domain of human CACNA1D (Cav1.3) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 98% identity with mouse and rat sequence.

Format: Lyophilized

Description: Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Subcellular location: Membrane; Multi-pass membrane protein. Tissue specificity: Expressed in pancreatic islets and in brain, where it has been seen in hippocampus, basal ganglia, habenula and thalamus. No expression in skeletal muscle. Also known as: Voltage-dependent L-type calcium channel subunit alpha-1D, Voltage-gated calcium channel subunit alpha Cav1.3, Calcium channel, L type, alpha-1 polypeptide, CACH3, CACN4, CACNL1A2, CCHL1A2, Cav3.1.

Specificity: Appears to be specific for CACNA1D.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Williams, Feldman, McCue et al.: "Structure and functional expression of alpha 1, alpha 2, and beta subunits of a novel human neuronal calcium channel subtype." in: Neuron, Vol. 8, Issue 1, pp. 71-84, 1992 (PubMed).

Seino, Chen, Seino et al.: "Cloning of the alpha 1 subunit of a voltage-dependent calcium channel expressed in pancreatic beta cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 2, pp. 584-8, 1992 (PubMed).

Kamp, Mitas, Fields et al.: "Transcriptional regulation of the neuronal L-type calcium channel alpha 1D subunit gene." in: Cellular and molecular neurobiology, Vol. 15, Issue 3, pp. 307-26, 1995 (PubMed).

Yamada, Masuda, Li et al.: "The structures of the human calcium channel alpha 1 subunit (CACNL1A2) and beta subunit (CACNLB3) genes." in: Genomics, Vol. 27, Issue 2, pp. 312-9, 1995 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).